安徽省淮南市首起0139霍乱弧菌疫情检测分析  

Experimental analysis the first cases of cholera epidemic detection of 0139 of Huainan

作　　者：余寿杰[1] 刘笑梅[1] 曹敏[1] 胡守奎[2] 胡万富[2] 

机构地区：[1]淮南市疾病预防控制中心,安徽省淮南232000 [2]安徽省疾病预防控制中心

出　　处：《中国基层医药》2011年第16期2166-2167,共2页Chinese Journal of Primary Medicine and Pharmacy

摘　　要：目的探讨淮南市首起0139霍乱弧菌疫情的发生原因和生物学特性。方法血清学及常规细菌学方法对菌株进行鉴定，聚合酶链式反应（PCR）检测霍乱毒力基因，改良K—B药敏实验。结果（1）培养分离及血清凝集试验结果，2例患者粪便标本全部阳性，密切接触者6人肛拭子全部阴性；甲鱼塘水样18份，阳性5份；甲鱼产蛋沙场样9份，2份阳性；甲鱼蛋7份，阳性3份；甲鱼排泄物11份，均为阴性。（2）血清凝集试验阳性培养物对头孢哌酮、强力霉素耐药；对氨苄西林、四环素中敏；对氟哌酸、庆大霉素、环丙沙星等敏感。（3）血清凝集试验阳性培养物霍乱毒素（CT）、毒素协同调节菌毛（TCP）全部阳性。结论该起0139霍乱弧菌疫情是因甲鱼养殖塘水污染引起，首例患者分离株与该患者所食甲鱼分离株PFGE带型一致，该菌种对头孢哌酮、强力霉素耐药并携带eta、tcpa毒力基因。Objective To identify the first cases of cholera in Huainan,0139 epidemic causes and biological properties. Methods Conventional methods of serological and bacteriological methods of the strains were identified by polymerase chain reaction （PCR） to detect cholera virulence genes, susceptibility testing modified KB. Results 1 ,was isolated and serum agglutination results,2 patients were all positive stool samples,6 were in close contact with all the negative rectal swab;turtle pond-like 18,5 were positive; turtle egg in battle were like 9,2 were positive ; turtle eggs 7, the positive three copies; turtle waste 11, were negative. 2, or more positive cuhure drug sensitiv- ity test of the pioneer B, doxycycline resistant; to ampicillin, tetracycline in sensitive; on norfloxacin, gentamicin, cipro- floxacin sensitive. 3 ,or more positive cultures gene DNA PCR test results ,cholera toxin（ CT）, toxin coregulated pilus （TCP） all were positive. Conclusion From the outbreak of cholera 0139 is water pollution caused by turtle ponds, The trip types of 0139 Vibrio cholera were highly homologous with that isolated from green turtle; the bacteria of the pioneer B, doxyeycline resistance and carrying eta, tcpa virulence genes.

关 键 词：霍乱 霍乱毒素 协同调节菌毛 甲鱼 

分 类 号：R51[医药卫生—内科学]