化学发光检测梅毒特异性抗体反应性结果分析  

Reactive results of Treponema pallidum antibody in chemiluminescent assay

作　　者：顾春瑜[1] 刘刚[1] 赵乔妹[1] 李少增[1] 于勇[1] 

机构地区：[1]解放军总医院第一附属医院检验科,北京100048

出　　处：《武警医学》2011年第8期676-678,共3页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的分析梅毒特异性抗体(treponema pallidum antibody,TP)的有反应性结果,尤其是COI值较低的标本,为减少假阳性的发生提供依据。方法选取129例用化学发光法检测梅毒特异性抗体有反应性的术前筛查标本,用梅毒螺旋体血凝试剂盒(treponema pallidum hemaglutination assay,TPHA)进行复检,分析比对两者的结果。结果当切点S/CO值为5时,Syphilis TP阳性为96例,阴性为33例,两种方法差异无统计学意义(P>0.05)。结论当TP有反应性时(尤其是S/CO值为1.0～5.0的标本),要综合考虑患者的年龄、病史,以及临床表现,可再用TPHA做复检,如两者均为阳性再报阳性,这样大大减少了假阳性的发生,降低了医疗纠纷的可能。Objective To find the cause of false positive results during the detection of Treponema pallidum antibody（TP）in chemilumineseent assay. Methods We put 129 TP reactive samples to ehemiluminscent assay, reexamined them by treponema palli- dum hemaglutination assay （TPHA） before comparing them. Results When cut - off S/CO was 1, the difference was significant, but there was no significant difference when cut - off S/CO was 5. Conclusions When anti - TP is reactive, especially in the samples of low S/CO value（ 1.0 -5.0） , multiple factors should be taken into consideration, such as age, history of disease, and clinical symp- toms , followed by re - examination of the sample by other means. In this way, the chance of false positivity will be reduced.

关 键 词：梅毒特异性抗体 反应性 假阳性 

分 类 号：R466[医药卫生—临床医学]