应用EP-7A2文件评价三酰甘油对神经元特异性烯醇化酶的干扰  

Evaluation the Interference of Neuron-Specific Enolase by Triglyceride Using EP-TA2 Files

作　　者：黄惠[1] 李强[1] 欧财文[1] 王志银[1] 

机构地区：[1]广东省中医院检验科,广州510120

出　　处：《现代检验医学杂志》2011年第4期98-99,102,共3页Journal of Modern Laboratory Medicine

摘　　要：目的探讨不同浓度的三酰甘油（triglyceride，TG）对ELISA法检测神经元特异性烯醇化酶（neuron—specificeno-Iase，NSE）的干扰。方法依据CLSIEP-7A2文件，以电化学发光法为NSE检测的比较方法，ELISA法为NSE检测的被评价方法。①以NSE的偏差（被评价方法-比较方法）为Y轴，比较方法的NSE浓度为X轴，将20个测试标本和20个对照标本绘制偏差图，分析浓度为4mmol／L的TG对比较方法检测NSE（浓度范围在5～20ng／ml）的干扰．②再以TG浓度为X轴，其对应的NSE降低值为Y轴绘常JTG干扰NSE测定的剂量响应曲线。结果①浓度为4mmol／L的TG时ELISA法检测的NSE（浓度范围在5～20ng／m1）产生了负干扰。②通过绘制TG干扰NSE测定的荆量响应曲线发现随着TG浓度的增加，对浓度为5．4ng／ml和20ng／ml的NSE样本产生负干扰也增加。其剂量响应曲线呈线性关系，回归方程分另4为Y=-2．4X＋18．92和Y=-O．69X＋5．26。结论使用高浓度TG患者血清标本制备干扰物，当TG浓度为1～4mmol／L时，对ELISA法检测NSE有负干扰，干扰的剂量曲线呈直线相关。Objective To explore the different concentrations of triglyceride （TG） interfere neuron-specific enolase （NSE） assayed by ELISA method. Methods According to CLSI EP-7A2 file,using electrochemical luminescence as the comparative method and ELISA method as the evaluated method for NSE assay. （1）For NSE deviation （evaluated method-comparative method） as the Y axis,the concentration of the comparative method as the X-axis. 20 test samples and 20 samples were drawn the deviation map,comparative analysis of TG concentration between 1 and 4 mmol/L was detected by the interference of NSE（concentration between 5 and 20 ng/ml）. （2）Using TG concentration as the X-axis ,the decresing value of NSE as Y-axis drawing the dose response curves of TG interfering NSE assay. Results （1）TG concentration at 1-4 mmol/L has negative interference effect on NSE （concentration between 5 and 20 retool/L） assayed by ELISA. （2）The dose response curves of TG interfering NSE showed that the TG concentration increasing accompanied by negative interference increased when the concentration of NSE at 5 ng/ml and 20 ng/ml. The dose-response curve was linear regression and equation were as Y= -2.4X＋18.92 and Y= - 0. 69X＋5.26. Conclusion Using patient sera with high TG concentration for preparation of interference sera when the TG concentration between 1 mmol/L and 4 mmol/L,the assay of NSE has negative interference by ELISA,the interference dose curve were linear correlation.

关 键 词：干扰 神经元特异性烯醇化酶 三酰甘油 

分 类 号：R446.1[医药卫生—诊断学]