Annexin 5对雄性SD大鼠应激损伤的保护作用  

Protective effects of annexin 5 on stress injury in male SD rats

作　　者：林钗英[1] 时姗姗[1] 陶晓倩[1] 柳海燕[1] 靖俊[1] 姚兵[1] 

机构地区：[1]南京军区南京总医院解放军临床检验医学研究所生殖医学实验室,南京210002

出　　处：《临床检验杂志》2011年第5期381-383,共3页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金(30770801和81070480);全军"十一五"医药卫生科研基金(06H011)

摘　　要：目的研究膜联蛋白5(annexin 5,A5)对坐骨神经选择性损伤引起的慢性疼痛应激致雄性SD大鼠生殖损伤的保护作用。方法 32只雄性SD大鼠随机分为4组,分别建立对照组、假手术组、手术组和手术+A5组模型。手术+A5组于术后第2天腹腔注射15μg/kg剂量的A5,其他实验组腹腔注射等量的pH 8.0 Tris-HCl,每日1次,连续21 d。分别测各组SD大鼠体重、称重睾丸和附睾,计算其脏器系数,并对附睾尾进行精子计数。HE染色观察睾丸组织结构变化。用化学发光法检测各组大鼠血清中睾酮浓度。结果手术组大鼠与对照组相比,睾丸系数、附睾系数以及精子相对计数均降低(P<0.05);睾酮水平下降显著[(5.10±2.61)vs(22.40±4.30)mmol/L,P<0.01)];病理切片见睾丸生精细胞与腔内精子数量明显减少。假手术组大鼠与对照组比,上述指标均无统计学差异。手术+A5组与手术组比,精子相对计数与睾酮含量[(19.67±2.02)vs(5.10±2.61)mmol/L,P<0.01]均显著提高,其他参数均有不同程度的恢复,睾丸生精细胞与腔内精子数量减少程度显著恢复。结论 A5对慢性疼痛应激引起的生殖损伤具有一定的保护作用。Objective To study the protective effect of annexin 5 （ A5 ） on the stress-induced injuries in reproductive organ of male SD rats. Methods Thirty-two male SD rats were randomly divided into 4 groups, i. e. , control group, sham group, surgery group and A5 group. The rats of A5 group were intraperitoneally injected with 15μg/kg of annexin 5 per day, while the other groups were injected with equal volume of Tris-HC1 buffer for 21 days. The weights of testis and epididymis were measured and the amount of spermatozoon from the caudal epididymis was counted. The testis structure was observed by H-E stain. The testosterone level in serum was determined by chemiluminescence assay. Results Comparing with the control group, the coefficient of testis and epididymis, the amount of spermatozoon, and the testosterone level [ （ 5.10 ±2.61 ） vs （ 22.40±4.30） mmol/L, P 〈 0.01 ] in the surgery group were all decreased significantly. H-E stain also showed that the amount of spermatids and spermatozoon reduced obviously in surgery group. Comparing with the surgery group, the amount of spermatozoon and the level of testosterone [ （19.67 ±2.02 ） vs （5.10 ± 2.61 ） mmol/L, P 〈 0.01 ] in A5 group were increased significantly, and the pathologic changes of testis tissue was improved. Conclusion Annexin5 may protect reproductive organ from injuries induced by chronic pain stress.

关 键 词：膜联蛋白5 慢性疼痛应激 睾酮 神经损伤 大鼠 

分 类 号：Q492[生物学—生理学]