不同营养方式对肝硬化大鼠肝切除术后肝脏自然杀伤细胞的影响  

Effects of different nutrition methods on the number and activity of natural killer cells in fiver after hepatectomy in cirrhotic rats

作　　者：罗时敏[1] 刘建[1] 曹杰[2] 谭卫民[1] 罗建威[1] 

机构地区：[1]广州医学院附属广州市第一人民医院综合外科,510180 [2]广州医学院附属广州市第一人民医院普通外科,510180

出　　处：《中华临床营养杂志》2011年第4期255-258,I0005,共5页Chinese Journal of Clinical Nutrition

基　　金：广东省医学科学技术研究基金项目（A2007490）和广州市医药卫生科技项目（2007-YB-011）

摘　　要：目的探讨不同营养方式对肝硬化大鼠肝切除术后肝脏自然杀伤（NK）细胞数量和活性的影响。方法采用硫代乙酰胺诱导大鼠肝硬化模型。将20只肝硬化大鼠按随机号码表法随机分为肠内营养组和肠外营养组，每组10只，分别于肝切除术后1 d开始进行为期5 d的肠内营养和肠外营养。应用流式细胞术测定大鼠肝脏NK细胞百分率；4 h51Cr释放法测定肝脏NK细胞的杀伤活性。结果肠内营养组的肝脏NK细胞百分率（22.2%±1.7%比17.4%±1.8%，P=0.000）和NK细胞杀伤活性均明显高于肠外营养组（41.6%±2.3%比37.1%±2.2%，P=0.000）。结论肠内营养可以增加肝硬化大鼠肝切除术后肝脏NK细胞的百分率和杀伤活性。ObjectiveTo explore the effects of different nutrition methods on the number and activity of natural killer （NK） cells in liver after hepatectomy in cirrhotic rats. MethodsTwenty thioacetamide-induced cirrhotic rats were randomly divided into enteral nutrition （EN） group （n=10） and parenteral nutrition （PN） group （n=10）, in which EN or PN was administered for 5 consecutive days 1 day after hepatectomy. The percentage of the NK cells in livers was examined by flow cytometry. Activity of NK cells was measured by 4 h 51Cr-release assay. ResultsCompared with the PN group, the percentage （22.2 %±1.7% vs. 17.4%±1.8%, P=0.000） and the activity of NK cells （41.6%±2.3% vs. 37.1%±2.2%, P=0.000） in the livers were significantly higher in the EN group. ConclusionEN after posthepatectomy can ameliorate the percentage and the activity of NK cells in the livers in cirrhotic rats.

关 键 词：肝硬化 肝切除术 肠外营养 肠内营养 自然杀伤细胞 

分 类 号：R459.3[医药卫生—治疗学]