Study on Five Methods for Extracting DNA from Dried Butterfly Specimen  

5种方法对蝴蝶干标本DNA的提取效果研究(英文)

作　　者：乔枫 陈振宁 耿贵工[2] 李思未 

机构地区：[1]青海师范大学青藏高原环境与资源教育部重点实验室,青海西宁810008 [2]青海农林科学院作物育种栽培研究所,青海西宁810016

出　　处：《Agricultural Science & Technology》2011年第8期1121-1124,共4页农业科学与技术（英文版）

基　　金：Supported by Scientific Research Foundation for Doctors in Qinghai Normal University~~

摘　　要：[Objective]The aim was to find out the optimal methods for extracting DNA from dried butterfly specimen.[Method]A total of five methods including SDS method,SDS-mercaptoethanol-phenol method,CTAB method,saturated NaCl method,SDS-mercaptoethanol-chloroform methods were employed to extract DNA from dried butterfly specimen.[Result]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method were suitable for genomic DNA extraction from dried butterfly samples,and the DNA extracted can be successfully applied to the PCR amplification of butterfly mitochondrial COI,EF-1α and CytB genes.[Conclusion]SDS-mercaptoethanol-phenol method,saturated NaCl method and SDS-mercaptoethanol-chloroform method can be used to study the extraction of DNA from dried butterfly samples,which laid foundation for study on molecular phylogenetics of butterfly.[目的]研究蝴蝶干标本DNA的提取方法。[方法]采用5种不同的方法(SDS法、SDS-巯基乙醇-酚法、CTAB法、饱和NaCl法、SDS-巯基乙醇-氯仿法)对蝴蝶干标本DNA进行提取。[结果]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法适合蝴蝶干标本基因组DNA的提取,并且能成功地应用于蝴蝶线粒体COI、EF-1α和CytB基因的PCR扩增。[结论]SDS-巯基乙醇-酚法、饱和NaCl法和SDS-巯基乙醇-氯仿法可以用于研究蝴蝶干标本DNA的抽提,为蝴蝶的分子系统进化研究奠定了基础。

关 键 词：BUTTERFLY Dried specimens DNA extraction 

分 类 号：S682.31[农业科学—观赏园艺]