布鲁氏菌M5-90ΔvirB2基因缺失株免疫小鼠抗体及相关细胞因子的检测研究  

Change of Antibody and Effect of Related Cytokines for Mice Vaccined by the Brucella M5-90ΔvirB2 Vaccine Strain

作　　者：李臻[1] 陈创夫[2] 王远志[3] 葛阳春[2] 张红星[4] 杨明锋[2] 张辉[2] 

机构地区：[1]石河子大学生命科学学院,石河子832003 [2]石河子大学动物科技学院,石河子832003 [3]石河子大学医学院,石河子832003 [4]安阳市中医院,安阳455000

出　　处：《石河子大学学报（自然科学版）》2011年第4期452-455,共4页Journal of Shihezi University(Natural Science)

基　　金：国家“973”计划项目(2010CB30203);国家自然科学基金项目(30760187),国家自然科学基金项目(31060334),国家自然科学基金项目(30800813)

摘　　要：对构建的M5-90ΔvirB2基因缺失株进行免疫原性初步研究。分别用1×106 CFU剂量的M5-90ΔvirB2株与M5-90疫苗株接种BALB/c小鼠,SAT试验和RBPT试验验证体液免疫产生的抗体水平;用ELISA方法检测小鼠外周血细胞因子IL-2、IL-4、IL-6、IL-10、TNF-α。结果表明,抗体水平检测结果显示M5-90ΔvirB2弱于M5-90;细胞因子检测水平显示M5-90ΔvirB2也低于M5-90。本研究为布鲁氏菌疫苗株M5-90的改造奠定了基础,并提供了相关的理论依据。Research of immunogenicity of the M5-9OAvirB2 deletion mutants have been constructed. Respectively 1 × 10^6 CFU dose of M5-9OAvirB2 and M5-90 vaccine strains were inoculated BALB/c mice. Through the SAT and the RBPT validate humoral antibody level;Cytokine response （IL-2, IL-4, IL-6, IL-10, TNF-α） of peripheral blood of mice were tested by using ELISA. Antibody test results showed that the M5-9OAvirB2 were weaker than the M5-90;Cytokine test showed the same re- suits. The conclusion was that the study laid the groundwork and offered relative theory for reconstruction of vaccine of B. melitensis M5 strains in future.

关 键 词：布鲁氏菌 virB2 基因缺失株 抗体水平 细胞因子 

分 类 号：S855.12[农业科学—临床兽医学]