低温条件下木薯种茎发芽性能与生理指标相关性研究  

Relationship of germination performance and some physio-biochemical indices of cassava stems under low temperature

作　　者：刘惠杰[1] 禤维言[1] 叶艺[1] 葛玉红[1] 姬秋梅[1] 冯斗[1] 

机构地区：[1]广西大学农学院,南宁530005

出　　处：《南方农业学报》2011年第8期866-869,共4页Journal of Southern Agriculture

基　　金：国家科技支撑计划项目(2007BAD75B03;2008BADB8B01);广西科技攻关项目(桂科攻0895003-1-2)

摘　　要：【目的】探讨木薯在低温寒害下的发芽性能和生理生化特性,评选出与木薯寒害、冻害密切相关的生理指标。【方法】将木薯品种桂热3、华南124和华南205的种茎置于8、5、2℃3种低温胁迫下,分别胁迫处理0(CK)、2、4、6、8、10、12、14d,测定发芽率、相对电导率和脯氨酸含量等指标,并同时进行发芽试验。【结果】低温胁迫时,相同处理条件下木薯种茎发芽率由高到低为:华南124>华南205>桂热3号,与试验材料本身抗寒性特征一致;低温处理早期,桂热3相对电导率迅速增加,华南124的相对电导率增加较缓慢,华南205在2℃处理6d时相对电导率达6.0%;不同品种脯氨酸含量变快趋势也不一致,桂热3号、华南205和华南124分别在2℃处理4、6和12d时,种茎脯氨酸含量达80.0μg/gFW以上。【结论】相对电导率和脯氨酸含量可以作为评价木薯种茎抗寒性的参考指标。[Objective]The germination and some chilling injury related with physio-biochemical indices of cassava were studied in order to forecast for application of control measures, and to evaluate the varieties tolerance to cold temperature. [Method]Three cassava varieties, viz., Guire 3, Huanan 124 and Huanan 205 were treated with different low temperatures （2, 5 and 8℃） for 0（CK）,2, 4, 6, 8, 10, 12 and 14 d,respectively,in three replicates. The germination rate and some physio-biochemical indices such as relative conductivity and proline content, etc., were determined. [Result]Under the similar temperature conditions, the variety Huanan 124 showed maximum germination rate followed by the varieties Huanan 205 and Guire 3. However, the relative conductivity of Guire 3 increased sharply, compared to Huanan 124, which was comparatively slower at the early stage of the treatment. The relative conductivity of Hunan 205 treated for 6 d at 2℃ was over 6%. The proline content of Guire 3, Huanan 205 and Huanan 124 were found more than 80.0% in the treatments of 4, 6 and 12 d, respectively, at 2℃ temperature. [Conclusion]The relative conductivity and proline content may be used as physio-biochemical markers for evaluating cassava varieties against low temperature.

关 键 词：低温胁迫 木薯 相对电导率 脯氨酸 发芽率 

分 类 号：S533.01[农业科学—作物学]