克雷伯氏菌属降解羟肟酸捕收剂H_(205)的研究  

Research on Biodegradation of Hydroxamic Acid Collector H_(205) by Klebsiellasp

作　　者：许念[1] 龚文琪[1] 梅光军[1] 王灿[1] 

机构地区：[1]武汉理工大学资源与环境工程学院,武汉430070

出　　处：《武汉理工大学学报》2011年第8期108-112,共5页Journal of Wuhan University of Technology

基　　金：国家"863"计划(2007AA06Z123);污染物分析与资源化技术湖北省重点实验室开放基金

摘　　要：采用从汽车修理厂土壤中采集的菌种,借鉴国内外对多环芳烃化合物降解菌的驯化和筛选的方法,在自然条件下驯化、分离、筛选出能降解H205的菌株。研究各单因素对此优势降解菌的生物降解特性的影响,找出此优势降解菌的最佳降解条件。结果表明,从汽车修理厂土壤驯化而来的H205降解菌是革兰氏阴性菌,经16SrRNA鉴定为克雷伯氏菌属;该菌降解H205的最佳条件为:pH 6～7,温度30℃,摇床转速150r/min,细菌接种量10%;底物浓度100mg/L;外加碳源葡萄糖、蔗糖、乙酸钠都对H205的降解有抑制作用;外加氮源酵母膏、蛋白胨对H205的降解有促进作用。Bacteria collected from soil in a vehicle repair plant were used with the method in acclimatization and screening of bacteria for PHAs degradation home and abroad.Under natural conditions,strains that can degrade hydroxamic acid collector H205 were cultivated through acclimation,isolation and screening.And this paper studied the effect on this dominant bacteria＇s biological degradation to induce the best degradation conditions.The results show that the bacterium domesticated from a vehicle repair plant soil that can degrade H205 is a Gram-negative bacterium identified as Klebsiella sp by 16SrRNA method;The best degradation condition is： pH 6~7,temperature 30 ℃,rotation speed 150 r/min,inoculums 10%,and concentration of substrate 150 mg/L;External carbon sources such as glucose,sucrose,sodium acetate have negative effects on the degradation of H205;External nitrogen sources such as yeast extract and peptone have positive effects on the degradation of H205.

关 键 词：羟肟酸 H205 生物降解特性 

分 类 号：X703[环境科学与工程—环境工程]