卡维地洛对自身免疫性心肌炎的免疫调节作用  

Carvedilol protects against rat autoimmune myocarditis by modulates the cytokines

作　　者：刘慧[1] 刘佳[1] 黄巍[2] 陈琳[1] 王丽珍[1] 孔一慧[1] 谷文光[3] 

机构地区：[1]哈尔滨医科大学附属第一医院心血管内科,150001 [2]哈尔滨医科大学附属第三医院超声科,150040 [3]哈尔滨医科大学附属第一医院骨科

出　　处：《国际免疫学杂志》2011年第5期321-324,共4页International Journal of Immunology

基　　金：黑龙江省教育厅海外学人科研资助项目（1152hq29）

摘　　要：目的通过检测心肌组织中细胞因子表达量的变化来评价卡维地洛对大鼠实验性自身免疫性心肌炎（EAM）的治疗效果及其作用机制。方法大鼠接种肌球蛋白后被分为两组，治疗组每日给予卡维地洛，对照组给予赋形剂，治疗效果在第21天进行评价。结果实验组心肌炎症面积23．07％±1．6％与对照组33．65％±2．71％相比明显减少（P〈0．0001），左室短轴缩短率43．52％±3．53％与对照组32．3％±3．31％相比明显提高（P=0．0021）。治疗组中心力衰竭标示物心钠肽的表达量（7．28×10^6±0．49×10^6绝对复制数／总RNAμg）明显低于对照组（32．67×10^6±2．78×10^6绝对复制数／总RNAμg），（P〈0．0001）。治疗组炎性细胞因子IL-1β的表达量（0．298±0．04）明显低于对照组（0．818±0．252），（P=0．0005），而抗炎性细胞因子IL-1受体拮抗剂的表达量在治疗组0．112±0．009明显高于对照组（0．051±0．002），（P〈0．0001）。结论卡维地洛对EAM有良好的治疗效果，其治疗机制可能是通过抑制炎性细胞因子同时增加抗炎性细胞因子等免疫调节作用而发挥。Objective We investigated whether the therapeutic effects of carvedilol are exerted in experimental autoimmune myocarditis （EAM）. Methods After immunization, rats were orally administered either carvedilol or a vehicle control for 21days. The heart weight to body weight ratio, the area of myocarditis, echocardiography parameters and the gene expression of atrial natriuretic peptide （ANP）, IL-1β and IL-1 receptor antagonist （IL-1RA） were measured on day 21. Results Carvedilol was effective in controlling EAM, as apparent by a reduced area of myocarditis （23.07% ± 1.6% vs 33.65% ± 2.71% ; P 〈0.0001）, and reduced expression of the gene encoding ANP （7.28 × 10^6 ± 0.49 × 10^6 vs 32.67 ×10^6 × 2.78 × 10^6 copy / total RNA p.g;P 〈0. 0001 ）. Echocardiography revealed that carvedilol administration resulted in a significant improvement of total RNA μg; P 〈 0.0001 ）. Echocardiography revealed that carvedilol administration resulted in a left ventricular fractional shortening （42.52% ± 3.53% vs 32.3% ± 3.31% ; P =0.002）. The mRNA level of the IL-1β （0. 298 ± 0.04 vs 0.818± 0.252 ; P = 0. 0005 i was markedly decreased and the IL-1RA （0. 112 ±0.009 vs 0. 051 ± 0. 002; P 〈 0. 0001 ） was markedly increased in the carvedilol-treated group. Conclusions Carvedilol protects against acute EAM in rats. This cardioprotective effect may be attributed to the suppression of pro-inflammatory cytokines and promotion of anti-inflammatory cytokines.

关 键 词：卡维地洛 心肌炎 细胞因子 IL-1 

分 类 号：R542[医药卫生—心血管疾病]