白芍的中药代谢特征谱研究  

Studies on metabolism of Chinese materia medica of radix from Paeonia lactiflora Pall

作　　者：潘艳丽[1] 董玲[2] 刘洋[3] 石任兵[2] 徐暾海[3] 翟华强[4] 赵保胜[5] 方敏[3] 汪国鹏[3] 

机构地区：[1]中国中医科学院中医药信息研究所办公室,北京100700 [2]北京中医药大学中药学院,100102 [3]北京中医药大学中药化学系,100102 [4]北京中医药大学临床中药系,100102 [5]北京中医药大学科研实验中心,100029

出　　处：《国际中医中药杂志》2011年第9期798-802,共5页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目（项目编号：30801510）

摘　　要：目的探讨芍药Paeonia lactiflora Pall．的干燥根中紫外可检出成分在大鼠体内的代谢过程。方法①运用HPLC—DAD技术，建立白芍水提物化学成分特征谱，进行其中总体化学成分的并行即时监测；②采用门静脉采血、腹主动脉采血、离体外翻肠囊法，建立白芍水提物入血成分、血中成分及肠吸收成分特征谱；③在相同色谱条件下，分析比较大鼠门静脉入血成分、腹主动脉血中成分及肠吸收成分的化学成分特征谱，推断白芍水提取物中化学成分的代谢过程。结果在分析的24种成分中，白芍中的6种成分不能被肠直接吸收；8种成分可被肠道直接吸收入血；7种成分被肠道直接吸收入血前已被肠内菌群代谢；血中出现的8种成分在白芍水提取物中未能查见，推测其为肠代谢转化后新生的成分吸收入血。结论本实验可判定整体用药时白芍所含化学成分的代谢过程。Objective To research the metabolism of components in the Radix of Paeonia lactifloraPall. Methods① we established the HPLC fingerprint of water extract of Paeonia lactiflora Pall. and real-time monitored the chemical composition. ② We established the HPLC fingerprint of rats＇ serum samples from hepatic portal vein, serum samples from aorta abdominalis and samples of intestinal absorption of Paeonia lactiflora Pall. ③ On this basis, using the established methods, HPLC fingerprint spectrum of serum samples, the sample of herb, the sample after intestinal metabolism, rats＇ serum samples from hepatic portal vein and rats＇ serum samples from aorta abdominalis were analyzed and compared in order to infer the metabolism of components in the Radix of Paeonia lactiflora Pall. Results 24 compositions were detected, seven of which were metabolized by intestinal flora and could not be absorbed into blood; six of them could not be absorbed directedly into intestinal; eight new compounds were absorbed into blood after bowel metabolism while they were not detected in water extract in Paeonia lactiflora Pall. Conclusion We could infer the metabolic processes of chemicals of Paeonia lactiflora Pall. in oral administration with this method

关 键 词：白芍 中药代谢 特征谱 门静脉入血成分 腹主动脉血中成分 肠吸收成分 

分 类 号：R285[医药卫生—中药学]