用RP-HPLC法测定三利乳膏中盐酸利多卡因的含量  

Determination of lidocaine hydrochloride content in Sanli cream by RP-HPLC method

作　　者：陆松伟[1] 朱全刚[1] 陈方剑[1] 王景阳[2] 

机构地区：[1]第二军医大学长海医院药学部,上海200433 [2]第二军医大学长海医院麻醉科,上海200433

出　　处：《药学服务与研究》2011年第4期291-293,共3页Pharmaceutical Care and Research

摘　　要：目的：建立测定三利乳膏中盐酸利多卡因含量的RP-HPLC法。方法：色谱柱为Kromasil-C18（150 mm×4.6 mm,5μm）;流动相为乙腈-0.02 mol/L磷酸二氢钠-三乙胺（47∶53∶0.15）,用磷酸调节pH值至3.15,内含0.015 mol/L十二烷基硫酸钠（SDS）;检测波长220 nm;流速1.0 ml/min。结果：盐酸利多卡因在6.576－87.68μg/ml的线性关系良好（r=0.999 7,n=3）,低、中、高三种浓度的回收率分别为（99.78±1.14）%、（98.91±1.78）%、（100.77±2.32）%。结论：该方法简便、准确,重复性好,适用于三利乳膏的质量控制。Objective： To establish a RP-HPLC method for determination of lidocaine hydrochloride content in Sanli cream.Methods： The separation was performed on a Kromasil-C18 analytical column（150 mm×4.6 mm,5 μm） with the mobile phase consisting of acetonitrile-0.02 mol/L sodium dihydrogen phosphate-triethylamine（47∶53∶0.15,pH value was adjusted to 3.15 with phosphoric acid,including 0.015 mol/L sodium dodecyl sulfate）.The detection wavelength was 220 nm.The flow rate was 1.0 ml/min.Results： The standard curve was linear within the range of 6.576-87.68 μg/ml for lidocaine hydrochloride（r=0.999 7,n=3）.The average recoveries of low,middle and high concentrations were（99.78±1.14）%、（98.91±1.78）%,（100.77±2.32）%,respectively.Conclusion： The method is simple and accurate with a good reproducibility,and can be used as a quality control method for Sanli cream.

关 键 词：三利乳膏 盐酸利多卡因 含量测定 色谱法 高效液相 

分 类 号：R927.2[医药卫生—药学] R971.2