海洋枯草芽孢杆菌HS-A38产直链脂肽活性物质的初步检测  

Antimicrobial activity of liner lipopeptides produced by Bacillus subtilis HS-A38

作　　者：张欢[1] 丛丽娜[1] 侯英敏[1] 李爱民[1] 谢三群[1] 王丹[1] 

机构地区：[1]大连工业大学,辽宁大连116034

出　　处：《海洋科学》2011年第8期37-42,共6页Marine Sciences

基　　金：国家自然科学基金项目(31072224);辽宁省教育厅创新团队资助项目(LT2010012);辽宁省自然科学基金项目(20102009)

摘　　要：从海参肠道中分离得到了一株海洋枯草芽孢杆菌(Bacillus subtilis)HS-A38,将其发酵上清液经盐酸沉淀、有机溶剂萃取得到具有广谱抗菌活性的代谢物质。采用薄层层析和茚三酮显色检测到4种物质,分别命名为化合物1、2、3和4;应用层析板覆盖指示菌的生物自显影技术对其抗菌活性成分进行追踪、检测,结果发现化合物1和2具有显著抑制弧菌的作用。进一步检测分析表明,化合物1、2和4具有很强的热稳定性,初步推断它们属于直链的脂肽类化合物,并且这4种物质出现在不同的发酵代谢时期,这将为研究群体效应提供基础检测依据。A marine Bacillus subtilis strain HS-A38 was isolated from the sea cucumber intestine. The crude extract of fermentation broth was prepared by methods of hydrochloric acid precipitation and solvent extraction. The extract showed broad-spectrum antimicrobial activity using the paper disc agar diffusion method. Four components, named as compound 1, 2, 3, and 4 were found by the thin layer chromatography and ninhydrin color printing. The compounds with antimicrobial activity were detected and tracked by bioautography of Vibrio parahaemolyticus and triphenyltetrazolium chloride （TTC） coloration on the plate of thin layer chromatography. Two inhibition spots were found, corresponding to compound 1 and 2. The further study showed that the compound 1, 2, and 4 were stable at high temperature. Therefore, it was preliminarily deduced that the four compounds were all straight-chain lipopeptide produced at different phase of fermentation.

关 键 词：枯草芽孢杆(Bacillus subtilis) 抗菌活性 生物自显影 直链脂肽化合物 

分 类 号：S917.1[农业科学—水产科学]