基于二氧化硅球腔微电极阵列的过氧化氢生物传感器制备  

Fabrication of Hydrogen Peroxide Biosensors Based on Microelectrode Array of Silicon Dioxide Cavities

作　　者：周丽娟[1,2] 尹凡[2] 周宇[2,3] 

机构地区：[1]苏州大学医学部药学院,苏州215123 [2]江苏省新型功能材料重点建设实验室,常熟215500 [3]江苏大学化学化工学院,镇江212013

出　　处：《分析化学》2011年第9期1313-1317,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20975073);江苏省自然科学基金(No.BK2008147);常熟市2009年拔尖人才资助项目

摘　　要：以聚苯乙烯(PS)微球阵列为模板,采用溶胶-凝胶法在氧化铟锡(ITO)电极上制备了二氧化硅(SiO2)球腔阵列,扫描电镜显示此方法制备的SiO2球腔阵列高度有序。电化学研究结果表明,该球腔阵列的循环伏安曲线符合微电极阵列的电化学特点。将血红蛋白(Hb)作为氧化还原模型蛋白直接吸附于球腔内,制得电流型过氧化氢(H2O2)生物传感器,研究了Hb在该微电极阵列上的直接电化学和电催化性质。所构建的传感器对H2O2的响应快速灵敏,其线性范围为2.03×10-6～1.21×10-5mol/L和2.03×10-5～1.21×10-2mol/L;检出限为5.73×10-7mol/L,米氏常数为0.266 mmol/L。A silicon dioxide（SiO2） cavities array was fabricated on indium-tin oxide（ITO） electrode surface with the template of the polystyrene（PS） particles array by using sol-gel technique.The morphology of SiO2 cavities array was highly ordered which was obtained by scanning electrode microscope.The results of electrochemistrical study showed that the cyclic voltammetric（CV） curve of SiO2 cavities array was in accordance with that of microelectrodes array.Using hemoglobin（Hb） as model protein,an amperometric biosensor for detection of H2O2 was prepared by adsorbing Hb in SiO2 cavities directly.The properties of direct electrochemistry and electrocatalysis of Hb were studied by CV method.The response of the biosensor for H2O2 was fast.A linear relationship between current response and the concentration of H2O2 ranging from 2.03×10-6 to 1.21×10-2 mol/L was obtained with a detection limit of 5.73×10-7 mol/L.And the apparent Michaelis-Menten constant was 0.266 mmol/L.

关 键 词：溶胶-凝胶法 二氧化硅球腔 微电极阵列 血红蛋白 生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]