基于自动磁珠转运的转基因蛋白Cry1Ab检测  

Magnetic Beads Transfer Based Assay for Cry1 Ab Protein

作　　者：李冬阳[1] 樊凯[1] 吴坚[1] 应义斌[1] 

机构地区：[1]浙江大学生物系统工程与食品科学学院,杭州310029

出　　处：《分析化学》2011年第9期1318-1322,共5页Chinese Journal of Analytical Chemistry

基　　金：国家科技专项(No.2009ZX08012-004B)资助

摘　　要：基于自动磁珠转运,建立了转基因蛋白Cry1Ab免疫检测的新方法。利用水热法制备了粒径约400 nm的纳米磁球,并进行电镜表征,通过溶胶法对磁球表面进行氨基修饰,采用戊二醛偶联对磁珠实现抗体包被,在核酸提取仪中进行酶联免疫反应,采用分光光度法进行检测。本方法对转基因蛋白Cry1Ab的检出限低于1μg/L,与商品化酶联免疫试剂盒相当。本方法节约人力、严格控制时间,同时,所需设备成本较低,有望取代传统的手动检测技术。A novel method for the immunoassay of genetically modified organisms（GMOs） protein Cry1Ab was proposed.First the nano-magnetic beads with the diameters around 400 nm were prepared by hydrothermal method.Then,the prepared magnetic beads were modified with NH2 group using sol-gel approach.The antibodies were conjugated on the surface of magnetic beads through bi-functional agent glutaraldehyde.The immunoassay was carried out in the nucleic acid extractor which is commercial available.In this proposed method,the detection limit for Cry1Ab is less than 1 μg/L,which is comparable to the common ELISA methods.This automatic way is promising for its rapidity and simplicity.

关 键 词：转基因作物 磁分离 Cry1Ab蛋白 磁珠转运 免疫检测 

分 类 号：S188[农业科学—农业基础科学]