siRNA CCR7抑制乳腺癌细胞转移的体外实验研究  

siRNA CCR7 inhibition of metastasis of breastcancer cells in vitro

作　　者：余术宜[1] 吴文芳[2] 李建哲[3] 吉午阳[3] 陈玉祥[3] 

机构地区：[1]中南大学现代分析测试中心,湖南长沙410078 [2]石药集团中奇制药技术(石家庄)有限公司,河北石家庄050051 [3]中南大学药学院,湖南长沙410078

出　　处：《中国现代医学杂志》2011年第23期2834-2836,2841,共4页China Journal of Modern Medicine

摘　　要：目的探讨CCR7对肿瘤转移的影响。方法构建siRNA-CCR7载体,稳定转染表达CCR7的肿瘤细胞株MDA-MB-231,在mRNA和蛋白水平检测其对肿瘤细胞CCR7的抑制作用,再用趋化及侵袭实验检测其对肿瘤细胞趋化、侵袭能力的影响。结果稳定转染siRNA1、siRNA2的MDA-MB-231细胞,在mRNA、蛋白水平均能有效抑制肿瘤细胞CCR7表达,并能抑制CCL21刺激的趋化和侵袭能力。结论 siRNA-CCR7能有效抑制乳腺癌细胞MDA-MB-231的趋化和侵袭,该实验结果对进一步探讨CCR7在肿瘤转移中的作用、探讨CCR7化学抑制剂或中药成分具有重要启示作用。【Objective】 To study the function of CCR7 in tumor metastasis in vitro.【Methods】 Detected the expression of CCR7 in mRNA and protein levels in MDA-MB-231 cells stable transfected with siRNAs vectors targeted CCR7,then assessed the ability of chemotactic and invasive after the stable transfected cells stimulated by CCL21,through matrigel invasion assay.【Results】 Inhibition of CCR7 expression at the mRNA and protein levels could be detected in stable transfected cells,and the inhibiton of chemotactic and invasive responses stimulated by CCL21 also could be found in these cells.【Conclusions】 Inhibition CCR7 expression by asiRNA impairs chemotactic,invasion of MDA-MB-231 cells.It will lay a solid basis for us to further study the role of CCR7 in tumor metastasis and CCR7 specific inhibitor.

关 键 词：SIRNA CCR7 肿瘤转移 

分 类 号：Q813[生物学—生物工程]