电针足三里穴减轻烫伤大鼠小肠促炎细胞因子引起的病理损害  

Electro-acupuncture at Zusanli （足三里） alleviates pathologic injury induced by intestinal proinflamatory factors in scalded rats

作　　者：胡森[1] 王磊[1] 周国勇[1] 

机构地区：[1]解放军总医院第一附属医院烧伤研究所休克与多器官障碍实验室,北京100048

出　　处：《中国中西医结合急救杂志》2011年第4期216-218,共3页Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care

基　　金：军队“十一五”专项课题基金项目（062055）

摘　　要：目的研究电针足三里穴对烫伤大鼠小肠促炎细胞因子的抑制作用及其与胆碱能神经通路的关系。方法将40只Wistar大鼠按随机数字表法分为烫伤＋电针足三里组（烫伤电针组）、烫伤＋假电针组（烫伤假针组）、迷走神经切断＋烫伤＋假电针组（迷切烫伤假针组）和迷走神经切断＋烫伤＋电针足三里穴组（迷切烫伤电针组），每组10只。用沸水（96℃）浸泡15s造成大鼠背部35％总体表面积Ⅲ度烫伤。伤后20min，电针组持续针刺双侧足三里穴30min，强度为2～3mA，频率2～100Hz；假电针组采用相同频率和强度刺激非经非穴（足三里穴外侧旁开0．5cm）30min；迷走神经切断组先手术切断腹腔迷走神经再烫伤。各组大鼠于烫伤后6h处死，取空肠组织，用干湿重法测定小肠组织含水率，并检测肿瘤坏死因子-α（TNF—α）和一氧化氮（NO）含量以及一氧化氮合酶（NOS）、髓过氧化物酶（MPO）和二胺氧化酶（DAO）活性。结果与烫伤电针组比较，烫伤假针组、迷切烫伤假针组、迷切烫伤电针组TNF-α、NO含量、N0s、MPO活性和小肠组织含水率显著升高，DAO活性显著降低（均P〈0．01）。与烫伤假针组比较，迷切烫伤假针组、迷切烫伤电针组TNF—α水平显著升高，DA0活性显著降低（均P〈0．05），NO和N0s、MPO水平比较差异均无统计学意义（均P〉0．05），但迷切烫伤假针组与迷切烫伤电针组上述指标的变化差异均无统计学意义（均P〉0．05）。结论电针足三里穴可显著抑制烫伤大鼠肠道促炎细胞因子水平、减轻肠黏膜损伤和组织水肿，其作用机制可能与激活胆碱能神经通路有关。Objective To investigate the inhibitory effects of electro-acupuncture（EA） at Zusanli （足三里） on small intestinal proinflammatory factors and tissue insult in rats with scald injury and their relationship to cholinergic nervous pathway. Methods Forty Wistar rats were randomly divided into four groups （each, n= 10） ： scald ＋ EA group （S/EA group）, scald ＋ shame EA group （S/SEA group）, vagotomy ＋ scald ＋ shame EA group （VA/S/SEA group） and vagotomy ＋scald ＋EA group （VA/S/EA group）. The rat＇s back was subjected to boiling water （96℃） for 15 seconds to produce a 35% full thickness scald injury of total body surface area （TBSA）. Twenty minutes after scald, bilateral Zusanli points were persistently electroacupunctured with constant voltage （2 - 3 mA, 2 - 100 Hz for 30 minutes ）. The shame EA group was performed at a point 0.5 cm lateral to the Zusanli point with the same frequency and intensity of stimulation for 30 minutes. Abdominal vagotomy was performed in rats in VA/S/SEA and VA/S/EA groups before scald. All the rats were sacrificed 6 hours after scald, and the specimens of jejunum were harvested to evaluate the activities of tumor necrosis factor-α （TNF-α）, nitric oxide （NO）, nitric oxide synthase （NOS）, myeloperoxidase （MPO）, diamine oxidase （DAO） and assessment of the rate of water content （ratio of dry to wet weight）. Results Compared to the S/EA group, the levels of TNF-α and NO, activities of NOS and MPO and the rates of water content in jejunum were significantly higher and the activity of DAO was obviously lower in S/SEA, VA/S/SEA and VA/S/EA groups 6 hours after scald （all P〈0.01）. Compared to the S/SEA group, the level of TNF-α was markedly higher and of DAO obviously lower in VA/S/SEA and VA/S/EA groups （all P〈0. 05） ; the comparisons of levels of NO,NOS and MPO did not reveal any statistical significant differences （P〉0.05）. In regard to the changes of all the above indexes, no statistical diffe

关 键 词：电针 足三里 烫伤 小肠 炎症 

分 类 号：R245[医药卫生—针灸推拿学] R644[医药卫生—中医临床基础]