马铃薯病毒的RT-PCR检测技术评述  

RT-PCR Detection Technology for Potato Viruses

作　　者：吴兴泉[1] 时妍[1] 杨庆东 李桂玲[1] 

机构地区：[1]河南工业大学生物工程学院,河南郑州450001 [2]北大荒薯业集团公司九三分公司,黑龙江嫩江161441

出　　处：《中国马铃薯》2011年第4期251-254,共4页Chinese Potato Journal

基　　金：河南省教育厅自然科学基础研究项目(2006210003);河南工业大学校科研基金项目(07XJC008)

摘　　要：对应用于马铃薯病毒检测中9种不同形式的RT-PCR检测技术进行评述,重点讨论常规RT-PCR、多重RT-PCR、Real-time RT-PCR等方法。分析了RT-PCR在同种病毒不同株系鉴定中的应用效果及建立系统进化树时应注意的问题。对RT-PCR技术在马铃薯病毒检测中可能出现的问题也进行了分析,并提出了应对策略。Nine kinds of potato viruse RT-PCR detection methods were reviewed,and the conventional RT-PCR,the multiple RT-PCR and real-time RT-PCR were discussed in details.The identification effect of RT-PCR on different strains of one virus and problems of the phylogenetic tree construction were analyzed.The problems in potato virus RT-PCR detection technology were also analyzed,and some countermeasures for improving were put forward accordingly.

关 键 词：马铃薯病毒 RT-PCR 检测 问题与对策 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]