废次烟叶绿原酸提取物的纯化  

Purification of Chlorogenic Acid in Extracts of Waste and Inferior Tobacco Leaves

作　　者：古君平[1,2] 魏万之[1] 

机构地区：[1]湖南大学化学生物传感与计量学国家重点实验室,长沙市岳麓区麓山南路410082 [2]广东中烟工业有限责任公司技术中心,广州市中山七路333号510145

出　　处：《烟草科技》2011年第8期52-54,共3页Tobacco Science & Technology

摘　　要：考察了聚酰胺树脂层析时上柱液浓度、洗脱剂浓度与用量及乙酸乙酯萃取时料液pH值、萃取次数与时间对废次烟叶中绿原酸纯化效果的影响。结果表明:①19.8 mL聚酰胺树脂玻璃柱(16 mm×200 mm)层析的适宜条件为:上柱液浓度8.5 mg/mL,洗脱剂198.0 mL 25%乙醇;②萃取条件为:料液pH2.0,萃取2次,萃取时间5 min。采用上述条件,可得到纯度82.1%的绿原酸产品,得率为57.6%。The effects of experimental conditions, such as injected solution concentration, concentration and volume of elution solution, for column chromatography, and sample solution＇s pH value, extracting time and number of repetitions on the purification of chlorogenic acid in extracts of waste and inferior tobacco were investigated. The results showed that： 1） the proper conditions of column （glass column 16 mm×200 mm, bed volume 19.8 mL） chromatography by polyamide resin were： concentration of crude chlorogenic acid solution 8.5 mg/mL, elution solution 198.0 mL 25% ethanol; 2） the appropriate conditions of extraction with ethyl acetate were： pH value of sample solution 2.0, extracting twice, extraction time 5 minutes. Under the mentioned conditions, the content of chlorogenic acid in the obtained product reached 82.1% with the yield of 57.6%.

关 键 词：废次烟叶 绿原酸 聚酰胺树脂 乙酸乙酯 

分 类 号：TS411.1[农业科学—烟草工业]