中国大陆汉族肾移植受者CYP3A5和MDR1基因多态性研究  

Study on the Hans renal transplant recipients' gene polymorphisms of CYP3A5 and MDR1 in China

作　　者：王彦斌[1] 李强[2] 林国跃[1] 于立新[2] 孟伟[1] 韩国林[1] 武阳[1] 

机构地区：[1]解放军第474医院,乌鲁木齐830013 [2]南方医科大学南方医院

出　　处：《山东医药》2011年第32期16-19,119,共5页Shandong Medical Journal

基　　金：兰州军区医药卫生科研计划项目(CWS10JA10)

摘　　要：目的建立细胞色素P450-3A5(CYP3A5)和多药耐药基因1(MDR1)3435、2677、1236基因多态性检测方法,并研究中国大陆汉族肾移植受者其基因多态性分布情况。方法自行设计引物,采用PCR法一次性扩增目的DNA,变性高效液相色谱技术检测分型。对在解放军第474医院肾移植中心及南方医院器官移植中心行肾移植手术及复诊的274例来自中国大陆12个省份的汉族肾移植受者CYP3A5和MDR1 3435、2677、1236基因多态性及分布情况进行分析,并观察是否存在省份差异,统计学方法采用多个独立样本比较的χ2检验。结果中国大陆汉族肾移植受者存在CYP3A5*3、MDR1 3435C>T、MDR1 2677G>T/A、MDR1 1236C>T基因变异,其变异频率分别为74.1%、49.5%、39.9%/13.7%、72.1%,不同省份间无统计学差异。结论中国大陆不同省份间汉族肾移植受者CYP3A5*3、MDR1(3435、2677、1236)基因变异无明显差异,但对于遗传基因相对较纯的小群体汉族和少数民族而言,仍有深入研究的意义。Objective To establish the detect method of gene polymorphisms of CYP3A5 and MDR1（3435,2677 and 1236）,and study their distributions in Hans renal transplant recipients in China.Methods We designed the primers and amplified the objective DNA by PCR at the same time,then detected the gene polymorphisms of CYP3A5 and MDR1（3435,2677 and 1236） with DHPLC.The clinical data consisted of 274 Hans renal transplant recipients from 12 provinces in China,whose distributions of CYP3A5 and MDR1（3435,2677 and 1236） were analyzed by χ2 test.Result The genic mutations of CYP3A5＊3,MDR1 3435CT,MDR1 2677GT/A and MDR1 1236CT existed in Hans renal transplant recipients in China,whose frequencies were as follows 74.1%,49.5%,39.9%/13.7% and 72.1%,and their distributions between different provinces had no statistical distinctions.Conclusion There are no differences in genic mutations of CYP3A5 and MDR1（3435,2677 and 1236） in Hans renal transplant recipients in different provinces of China,but the research should be lucubrated in Hans of pure genetic gene and minority groups.

关 键 词：细胞色素P450-3A5 多药耐药基因1 基因多态性 肾移植 人种群 

分 类 号：R617[医药卫生—外科学] R392.4[医药卫生—临床医学]