槟榔果实中的细胞毒活性成分研究  

Study on Cytotoxic Chemical Constituents in Areca catechu

作　　者：吴娇[1,2] 王辉[2] 李小娜[2] 梅文莉[2] 戴好富[2] 

机构地区：[1]海南大学农学院,海口570228 [2]中国热带农业科学院热带生物技术研究所海南省黎药资源天然产物研究与利用重点实验室,海口571101

出　　处：《河南大学学报（自然科学版）》2011年第5期511-514,共4页Journal of Henan University:Natural Science

基　　金：中央级科研院所基本科研业务费专项资金资助项目(ITBBZD2007-4-1)

摘　　要：采用多种柱色谱技术对槟榔(Areca catechu Linn.)果实中的化学成分进行分离纯化,通过波谱分析及文献数据对照鉴定其化合物的结构,并采用MTT(methyl thiazolyltetrazolium)法进行细胞毒活性测试.结果显示:从槟榔果实中分离得到2个化合物,分别鉴定为巴西红厚壳素(1)和环―(亮氨酸―酪氨酸)(2).化合物2为首次从槟榔果实中分离得到.化合物1对人肝癌细胞(SMMC-7721)和人胃癌细胞(SGC-7901)的增殖均显示了较强的生长抑制活性.化合物2对慢性髓原白血病细胞(K562)的增殖具有一定的生长抑制活性.The constituents in Areca catechu nuts were isolated by column chromatography,and their structures were identified by the spectroscopic evidences of UV,NMR and MS.Their cytotoxicity was tested by MTT assay.Two compounds were obtained from ethyl acetate extract and identified as jacareubin（1） and cyclo-（Leu-Tyr）（2）.Compound 1 showed significant inhibitory activities towards human gastric carcinoma（SGC-7901） and human hepatoma（SMMC-7721）.Compound 2 showed moderate inhibitory activity towards chronic myelogenous leukemia（K562） and was reported from Areca catechu for the first time.

关 键 词：槟榔 果实 化学成分 细胞毒活性 

分 类 号：R917[医药卫生—药物分析学]