三维荧光二阶校正测定细胞培养基样中山姜素  

Determination of Alpinetin in Cell Culture Medium Samples by Using Three-dimensional Fluorescence Coupled with Second-order Calibration

作　　者：吴海龙[1] 张娟[1] 王建瑶[1] 邵圣枝[1] 张晓华[1] 俞汝勤[1] 

机构地区：[1]湖南大学化学化工学院,化学生物传感与计量学国家重点实验室,湖南长沙410082

出　　处：《湖南大学学报（自然科学版）》2011年第8期50-54,共5页Journal of Hunan University:Natural Sciences

基　　金：国家自然科学基金资助项目(20775025);教育部长江学者和创新团队发展计划基金资助项目

摘　　要：建立了细胞培养基样中山姜素的定量测定方法.将三维激发发射荧光分别与化学计量学中基于平行因子分析(PARAFAC)算法和自加权交替三线性分解(SWATLD)算法的二阶校正方法相结合,当选取待分析体系组分数为2时,得到细胞培养基样中山姜素的平均回收率分别为(100.3±2.2)%,(100.1±2.2)%.两种算法所得检出限各为0.182 3μg.mL-1,0.178 1μg.mL-1.椭圆置信区间(EJCR)测试证明两种算法所得结果准确可靠.A new spectrofluorimetric method has been developed for the quantitative determination of the alpinetin content in cell culture medium samples. This method combines excitation-emission matrix fluorescence with chemometric second-order calibration methods based on Parallel Factor Analysis （PARAFAC） and Self-weighted Alternating Trilinear Decomposition （SWATLD） algorithms. When the component number was chosen to be 2, the average recoveries obtained were （100. 3 ± 2. 2）% for PARAFAC and （100. 1±2.2）% for SWATLD, respectively. The limits of determination for alpinetin were 0. 182 3μg·mL-1 for PARAFAC and 0. 178μg·mL-1 for SWATLD. EJCR has demonstrated that the results are accurate.

关 键 词：平行因子分析算法 山姜素 细胞培养基 三维荧光 二阶校正 自加权交替三线 性分解算法 

分 类 号：O657.3[理学—分析化学]