鄂柑一号椪柑少核芽变的AFLP分析  

Identification of Three Few-seed Mutant from E-Gan No.1 by AFLP

作　　者：许淼[1] 蒋迎春[1] 孙中海[1] 吴黎明[1] 仝铸[1] 王志静[1] 何利刚[1] 

机构地区：[1]湖北省农业科学院果树茶叶研究所,武汉430209

出　　处：《湖北农业科学》2011年第17期3556-3559,共4页Hubei Agricultural Sciences

基　　金：湖北省农业科技创新中心资助项目(2007-620-005-03-02);湖北省重点基金项目(2009CDA093);湖北省重大科技专项(2006AA201A206)

摘　　要：利用AFLP技术,对鄂柑一号椪柑(Citrus reticulata Blanc.cv.ponkan)的3个少核芽变及其母株进行分子鉴定。筛选了64对引物,有60对引物可扩增出清晰的条带,共获得2 281条清晰的扩增谱带,其中有9对引物可扩增出明显多态性条带,多态性位点共15个,占总数的0.66%。结果表明,鄂柑一号椪柑的少核芽变由遗传物质改变引起,通过AFLP技术可以有效鉴定。Three few-seed bud mutations and their mother plant ＇E-Gan No.1＇ ponkan were identificated by AFLP technique.64 selective primer combinations were tested and 2 281 clear bands were abtained from 60-pair-primer,from which 9-pair-primer were selected for further reseach.And 15 polymorphic bands were dectected out of 2281 total clear bands;the polymorphic rate was 0.66%.This results indicated that there were some molecular level differentiation between E-gan No.1 and the three few-seed bud mutations;and the differentiation could be identificated by AFLP.

关 键 词：椪柑(Citrus RETICULATA Blanc.cv.ponkan) 芽变 AFLP 

分 类 号：S666.1[农业科学—果树学]