条斑星鲽染色体带型研究  

Study on chromosome banding patterns of Verasper moseri

作　　者：王妍妍[1] 柳学周[1] 徐永江[1] 刘新富[1] 

机构地区：[1]农业部海洋渔业资源可持续利用重点开放实验室中国水产科学研究院黄海水产研究所青岛市海水鱼类种子工程与生物技术重点实验室,266071

出　　处：《渔业科学进展》2011年第4期6-13,共8页Progress in Fishery Sciences

基　　金：国家鲆鲽类产业技术体系建设项目(CARS-50);国家"863"计划项目(2006AA10A414;2010AA10A402);公益性农业行业专项项目(ny-hyzx07-046)

摘　　要：开展了条斑星鲽染色体C-带、Ag-NORs带及G-带的研究。结果发现,条斑星鲽染色体显示较为复杂的C-带,有4对染色体整体呈阳性深染,分别为3、13、20、22号染色体;3对具居间C-带,分别为1、4、8号染色体;9号染色体整体显示阴性,其余有大小不一的着丝粒C-带。计算其异染色质含量为54.91%。银染分裂相中Ag-NORs出现的数目以两个的频率最高,达87.5%,与此相一致,在间期核中通过银染表现出两个核仁的间期核数目的频率也最高,故确定条斑星鲽染色体具有1对Ag-NORs,位于亚中部着丝点染色体的短臂上,为端部Ag-NOR。Ag-NORs的数目为1~3个,以出现两个Ag-NOR的频率最高,达87.5%,与此相同的是,在间期核中,通过银染表现出两个核仁的间期核数目的频率也最高。G-带研究显示,条斑星鲽有37条深染带,22条浅染带。The C-binding,Ag-NORs,and G-banding patterns of the metaphase chromosomes of barfin flounder Verasper moseri were investigated in this study.The results showed that the interstitial regions of most chromosomes exhibited positive C-bands,in which the whole 3rd,13th,20th,and 22nd pair of chromosomes showed totally positive C-band,whilst the 1st,4th and 8th pair of chromosomes only showed positive C-bands in the middle region,the rest with centromeric positive C-bands.However,the 9th pair of chromosomes showed negative result.The C-banding heretochromatin occupied 54.91% of the total chromosome in the present study.Silver staining study showed that most chromosomes featured two silver-stained nuclear organizing regions（Ag-NORs） with the percentage of 87.5%.Accordingly,the interphase nucleus with 2 nucleolus appeared most frequently,thus it is supposed that the barfin flounder bears a pair of Ag-NORs which located at the end of short arm of the submetacentric chromosomes.A total of 59 positive G-bands（37 strong,22 light） were recorded in 23 pairs of chromosomes.These results would provide basic data for the genomic research and biodiversity conservation of barfin flounder.

关 键 词：条斑星鲽 C-带 AG-NORS带 G-带 

分 类 号：Q343.2[生物学—遗传学] Q959.4