揉髌手法对兔膝关节软骨增殖细胞核抗原表达的影响  

The Influence Of The Rou-Bin Manipulation To The PCNA Expression With Knee Joint Cartilage In Rabbit

作　　者：戴七一[1] 阮萍[1] 袁经阳[1] 覃学流[1] 黎强[2] 滕居赞[1] 容向宾[1] 韩杰[1] 林光琪[2] 刘靖[2] 覃杰[1] 王雄[1] 吴兆沛[1] 

机构地区：[1]广西中医学院附属瑞康医院,广西南宁530011 [2]广西中医学院,广西南宁530001

出　　处：《中医正骨》2011年第8期6-8,12,共4页The Journal of Traditional Chinese Orthopedics and Traumatology

基　　金：国家自然科学基金资助项目(编号:30860359);广西科技厅自然基金资助项目(编号:0832178)

摘　　要：目的:观察揉髌手法对兔膝关节软骨增殖细胞核抗原表达的影响,进而探讨揉髌手法治疗膝关节骨性关节炎的可能作用机制。方法:采用新西兰兔50只,随机分为手法组、针剂组、模型对照组、假模组和正常组各10只。前3组通过手术结扎并切断右侧臀下静脉、股静脉和大隐静脉造模,假模组显露相应血管但不结扎,正常组不做任何处理。造模1周后手法组采用揉髌手法分别施术于兔右下肢5周共17次;针剂组右膝关节内每周注射1次玻璃酸钠注射液,连用5周。其余3组不做任何处理。造模后8周末处死所有动物,取右膝关节内侧胫骨平台软骨组织,采用免疫组化法检测软骨细胞增殖率,对所得数据进行统计学处理。结果:模型组软骨细胞减少,增殖率为(13.25±2.66)%;手法组软骨细胞增多,增殖率为(17.90±2.80)%;针剂组软骨细胞增多,增殖率为(17.13±4.09)%;假模组软骨细胞增多,增殖率为(12.63±2.92)%;正常组软骨细胞正常,增殖率为(7.75±1.39)%;与模型组比较,手法组、针剂组和正常组P<0.01,或P<0.05,差异有统计学意义。结论:揉髌手法可促进兔膝关节软骨细胞增殖,降低软骨组织的损伤。Objective：To observe the Influence Of The Rou-Bin Manipulation To The PCNA Expression With Knee Joint Cartilage In Rabbit,explore the mechanism of action of Rou-Bin manipulation to the knee osteoarthritis.Methods：50 New Zealand rabbits devided randomly into five gourps： Manipulation group,Inject group,Model group,dummy-model group and the normal group,10 rabbits in each group;ligaturing and cutting off the inferior-gluteal vein,femoral vein and great saphenous vein on the right side of the rabbit for the first 3 groups,shows the same veins without cutting off for dummy-model group,no treatment for the normal group.1 week after modeled,17 times of Rou-Bin manipulation on each rabbit in manipulation group,the rabbit injection with sodium hyaluronate 5 times in inject group,no special treatment for other rabbits.The 8th weekend after modeled,to take all the rabbit right knee medial tibial plateau cartilage tissue processing,detected chondrocyte proliferation rate by IHCA,statistics processing.Results：The amount of model group chondrocyte decreases,with a proliferation rate（13.25±2.66）%;The amount of manipulation group chondrocyte increase,with a proliferation rate（17.90±2.80）%;The amount of inject group chondrocyte increase,with a proliferation rate（17.13±4.09）%;The amount of dummy-model group chondrocyte increase,with a proliferation rate（12.63±2.92）%;The normal group remain appro the same level,with a proliferation rate（7.75±1.39）%;To compare with model group,P0.01（or P0.05） for all of the manipulation group,inject group and normal group,has the highly significance difference.Conclusion：The Rou-Bin manipulation promote the chondrocyte proliferation rate of rabbit knee joint increase,reduce cartilage tissue damage.

关 键 词：手法 增殖细胞核抗原 膝关节 按摩 模型 动物 兔 

分 类 号：R244.1[医药卫生—针灸推拿学] R274.9[医药卫生—中医临床基础]