不同价态外源硒对冬小麦生长及生理代谢的影响  

Effects of Exogenetic Selenite and Selenate on the Growth and Physiological Metabolism of Winter Wheat

作　　者：付冬冬[1] 王松山[1] 梁东丽[1] 缪树寅[1] 段曼莉[1] 胡斌[1] 

机构地区：[1]西北农林科技大学资源环境学院,陕西杨凌712100

出　　处：《农业环境科学学报》2011年第8期1500-1507,共8页Journal of Agro-Environment Science

基　　金：国家小麦现代农业产业技术体系建设专项;西北农林科技大学"创新团队建设计划"

摘　　要：用盆栽试验研究了不同浓度外源硒(四价和六价)处理土壤对小麦生长和生理代谢的影响,旨在为富硒小麦的种植和开发提供理论依据。结果表明:外源硒抑制了苗期小麦的生长(株高和生物量);而在生长后期,随外源硒浓度增加表现为先促进后抑制作用。Se(Ⅳ)和Se(Ⅵ)处理对小麦生物量影响的EC10分别从苗期的1.40mg·kg-1和0.28mg·kg-1增加到收获期的28.48mg·kg-1和4.56mg·kg-1,表明小麦对硒的耐受浓度随生长时间的延长逐渐增大。Se(Ⅵ)低浓度(≤1mg·kg-1)对小麦生长的促进作用及高浓度(≥2.5mg·kg-1)对小麦生长的抑制作用均大于Se(Ⅳ)。小麦籽粒产量随外源Se(Ⅳ)和Se(Ⅵ)浓度的增大先升高而后降低,分别在2.5mg·kg-1和1mg·kg-1浓度处理时达到最大。低浓度硒处理(Se(Ⅳ)≤5mg·kg-1和Se(Ⅵ)≤0.5mg·kg-1)能促进拔节期小麦叶片中GSH和POD等抗氧化酶活性,显著降低叶片过氧化物质MDA(Se≤2.5mg·kg-1)含量,高浓度硒处理则与之相反。Pot experiments were conducted to investigate the effects of exogenetic selenite and selenate on the growth and physiological metabolism of winter wheat in order to provide theoretical basis for the planting of selenium-enriched wheat.The results showed that exogenetic selenium inhibited wheat seedling growth（plant height and biomass） at all selenium levels.However,selenium first stimulated and then inhibited wheat growth as the increase of exogenetic selenium concentration in the late growth stage.The EC10 for Se（Ⅳ） and Se（Ⅵ） increased from 1.40 mg·kg-1 and 0.28 mg·kg-1 at seedling stage to 28.48 mg·kg-1 and 4.56 mg·kg-1 at the harvest stage,respectively.Selenite had greater influence on the growth of wheat than selenate.Wheat yield first increased and then decreased as the increase of added selenium,and reached the maximum values at 2.5 mg·kg-1 for selenite and 1 mg·kg-1 for selenate treated plants.Physiological metabolism analysis of winter wheat leaves showed that lower concentration of selenium in soil（Se（Ⅳ）≤5 mg·kg-1 and Se（Ⅵ）≤0.5 mg·kg-1） stimulated the activity of GSH and POD,and decreased MDA（Se≤2.5 mg·kg-1） contents significantly;the contrary results were found for higher concentration selenium treated plants.

关 键 词：外源硒 生长 生理代谢 冬小麦 

分 类 号：X503.231[环境科学与工程—环境工程]