大黄苷元对不同时间溶栓治疗抗大鼠脑缺血损伤的比较研究  

Study of Rhubarb aglycone on time window of thrombolysis therapy through artery in rats with thrombus-occluded cerebral ischemia

作　　者：李建生[1] 刘敬霞[2] 王冬 梁生旺[1] 刘轲[1] 郭晓燕[1] 孙捷[1] 李宁[1] 苏静[1] 

机构地区：[1]河南中医学院老年医学研究所,郑州450008 [2]宁夏医科大学中医学院,银川750004 [3]河南省洛阳市妇女儿童保健中心儿童医院,洛阳471000

出　　处：《中华中医药杂志》2011年第9期1967-1971,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.30873265)~~

摘　　要：目的:比较不同时间经动脉溶栓治疗对血栓栓塞性脑缺血大鼠损伤的保护作用及大黄苷元对脑缺血溶栓治疗时间窗的影响。方法:大鼠随机分组。制备血栓栓塞性脑缺血动物模型。缺血后大鼠3、6、9h经导管区域动脉溶栓。动脉给药后24h,观察大鼠脑组织病理损伤及神经症状,测定脑组织含水量和脑梗塞面积变化,观察颅内出血率。结果:各模型组大鼠均较假手术组神经症状评分增高,脑组织含水量增加,脑梗塞面积增大,脑组织病理损伤明显;溶栓组和大黄苷元组各时间点大鼠神经症状评分降低、脑组织含水量减少、脑梗塞面积减小、脑病理损伤减轻;各用药3h组上述指标的改善均较9h组明显;溶栓组颅内出血率较模型组和大黄苷元组增高,联合组较溶栓组降低。结论:脑组织受损随缺血时间延长而加重;不同时间窗经动脉溶栓对脑缺血损伤均具有保护作用,但缺血后3h和6h溶栓效果明显优于9h;大黄苷元可增强溶栓效果,具有延长溶栓时间窗、降低溶栓后颅内出血率的作用。Objective：To compare the effects of thrombolysis therapy being used at different time after stroke through artery on rats with thrombus-occluded cerebral ischemia and then to discuss the influence of Rhubarb aglycone （RA） on time window of thrombolysis therapy. Methods： Rats in the study were randomly divided into several groups. Thrombus-occluded cerebral ischemia model group was duplicated by autologous blood blot and inserted nylon thread. Rats were administrated with thrombolysis therapy through artery at 3, 6, and 9h after cererbral ischemia. At 24h of administration through artery, rats neural symptoms evaluating score（NSES）, brain pathologic changes, fatality and intracranial hemorrhage ratio（ICHR） were observed, then measured brain water ratio（BWR） and cerbral infarction size（CIS）. Results： Compared with SH group, NSES, BWR and CIS increased in each model group, brain pathologic changes appeared more obviously. In thrombolysis group, NSES, BWR and CIS decreased, and brain pathologic changes abated, but rats fatality and ICHR increased in 9 h thrombolysis group. The improvement in associated group appeared more significant in comparison with that in thrombolysis group. Conclusion： Nerve functional impairment, brain dropsy and pathologic detriment in rats with cerebral ischemia exacerbated following the prolongation of ischemia. Thrombolysis therapy using at different time through artery all seemed protective, and effects appeared more significant within 6h of ischemia, delayed thrombolysis therapy could make rats fatality and ICHR increase. Rhubarb aglycone could increased the protection and decreased rats ICHR caused by delayed thrombolysis, performed prolongation of time window and decreased the ratio of intracranial hemorrhage after thrombolysis therapy.

关 键 词：脑缺血 大鼠 大黄苷元 溶栓 时间窗 

分 类 号：R285[医药卫生—中药学]