电针对雌性去势大鼠行为学和下丘脑室旁核c-fos表达的影响  

Effect of electroacupuncture on behavioral and the expression of proto-oncogene c-fos in the hypothalamic paraventricular nucleus in female ovariectomized rats

作　　者：申国明[1] 许浩[1] 胡光明[1] 尹刚[1] 王海颖[1] 刘艳[1] 

机构地区：[1]安徽中医学院中西医结合临床学院,合肥230038

出　　处：《中华中医药杂志》2011年第9期2062-2064,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81072871);安徽省教育厅自然科学重点科研项目(No.KJ2008A42ZC);安徽省自然科学基金(No.090413115)~~

摘　　要：目的:观察电针对雌性切除卵巢(去势)大鼠行为学、血浆雌二醇(E2)及下丘脑室旁核c-fos表达的影响,探讨电针的抗抑郁效应及其机制。方法:SPF级SD雌性成年大鼠40只,随机分为正常对照组、去势组、电针组及美雌醇组。除正常对照组外大鼠均行双侧卵巢切除术。2周后,给予双侧内关、三阴交穴位电针干预处理2周。强迫游泳实验(FST)观察行为学变化;免疫组化观察下丘脑室旁核c-fos表达;放射免疫方法检测血浆E2、FSH及LH变化。结果:大鼠卵巢切除4周后,FST显示不动时间明显增加(P<0.01),挣扎时间减少(P<0.05);血浆中E2水平降低(P<0.05),FSH(P<0.01)和LH(P<0.05)水平明显升高。与去势组比,电针组及美雌醇组不动时间明显减少(P<0.01,P<0.05),挣扎时间增加(P<0.05,P<0.01),血浆E2水平明显升高(P<0.01),FSH(P<0.05,P<0.01)和LH(P<0.01,P<0.05)水平明显降低,室旁核c-fos阳性表达明显增强。结论:去卵巢后,可致大鼠行为学改变,表现为抑郁倾向;电针干预具有明显抗抑郁效应,调整下丘脑轴是其可能机制。Objective： To observe the effects of electroacupuncture（EA） on depression-like behavior,estradiol（E2） and the expression of proto-oncogene c-fos in the hypothalamic paraventricular nucleus（PVN） in ovariectomized（OVX） female rats.Methods： SPF grade SD female rats were ovariectomized.After two weeks recovery,the rats in APU group were treated by EA in bilateral Neiguan and Sanyinjiao for two weeks and the rats in E group were treated by mestranol.Later let all the rats in every group to undergo FST.To measure the sum of time about immobility,struggling and swimming in the second FST（within 5 minutes）,the levels of E2,FSH,LH in blood serum were measured by Radioimmunoassay（RIA） and the expressions of proto-oncogene c-fos in PVN were ovserved by immunohistochemistry.Results： The time of immobility increased significantly in OVX group after rats were ovariectomized for 4 weeks（P〈0.01）,the levels of E2 in blood plasma decreased（P〈0.05）,and the levels of FSH（P〈0.01） and LH（P〈0.05） increased significantly.After treated by EA,the levels of E2 increased significantly（P〈0.01）,and the levels of FSH（P〈0.05） and LH（P〈0.01） increased significantly.Comparing with OVX group,the time of immobility in APU group and E group decreased significantly（P〈0.01,P〈0.05）,and the time of struggling increased（P〈0.05,P〈0.01）,the expressions of proto-oncogene C-fos＋ in PVN increased significantly.Conclusion： EA has an antidepressant effect and the system could be to regulate hypothalamus axis.

关 键 词：去势 强迫游泳 雌激素 下丘脑-垂体轴 

分 类 号：R245[医药卫生—针灸推拿学]