利开灵对脑出血大鼠内毒素血症炎症效应的影响  

Influence of Likailing Decoction on inflammatory effects of endotoxemia after intracerebral hemorrhage in rats

作　　者：傅晨[1] 张允岭[1] 常富业 刘雪梅[1] 王新祥[1] 郑宏[1] 陶冶[1] 闫妍[1] 黄启福[3] 

机构地区：[1]北京中医药大学东方医院实验中心,博士研究生100700 [2]煤炭总医院老年病科 [3]北京中医药大学基础医学院病理教研室

出　　处：《环球中医药》2011年第5期340-343,347,共5页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(30772807)

摘　　要：目的观察脑出血后大鼠继发内毒素血症的炎症效应,并探讨利开灵对其影响。方法采用Rosenberg法改良后建立大鼠脑出血模型,根据脑出血不同时点(12、24、48、72小时)分为4组,每个时间点再分为3组:假手术组、模型组、利开灵组,用终点显色法检测各组血浆内毒素(LPS)水平,ELISA法检测各组血清肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)水平,放射免疫法检测各组出血局部脑组织TNF-α、IL-1β水平。结果模型组LPS均明显升高,在48小时达高峰(P<0.01);血清TNF-α、IL-1β升高,均在24小时达高峰(P<0.01);出血局部脑组织TNF-α升高,呈上升趋势直至72小时,IL-1β呈先升高再下降的趋势,在48小时达最高(P<0.01)。同时发现利开灵可下调血浆LPS、血清和出血局部脑组织TNF-α、IL-1β过高表达。结论脑出血后大鼠继发内毒素血症,并且引发体内炎症反应和加重脑局部的炎症反应。利开灵对脑出血继发内毒素血症的炎症效应有较好的调控作用,这可能是利开灵改善脑出血后脑水肿的作用机制之一。Objective To observe the inflammatory effects of secondary endotoxemia after intracerebral hemorrhage in rats,and to explore the effect of Likailing Decoction（LKL）.Methods Rat models of intracerebral hemorrhage were established by improved Rosenberg method.Rats were randomly divided into sham group,model group and LKL treatment group.Observing the LPS content level in the plasma by endpoint chromogenic assay,the TNF-α、IL-1β content level in the serum by ELISA,the TNF-α、IL-1β content level in the surroundings of the bleeding region by radioimmunoassay at 12、24、48、72 hours.Results The LPS content in the plasma of model group rats obviously increased and reach the peak at 48h（P0.01）.The TNF-α、IL-1β content in the serum obviously increased and reach the peak at 24h（P0.01）.In the surroundings of the bleeding region,the TNF-α content level increased until 72h,and the IL-1β content level increased at first and then decreased,which peaked at 48h.Likailing Decoction could reduce the high expression of LPS in the plasma,the TNF-α、IL-1β in the serum,and the TNF-α、IL-1βin the surroundings of the bleeding region.Conclusion Intracerebral hemorrhage may cause endotoxemia in rats,and this may initiate inflammatory response in body and aggravate the inflammatory response in brain.Likailing Decoction has regulatory effect on inflammatory effects of endotoxemia after Intracerebral hemorrhage,which may be a part of the action mechanism of improving effect of Likailing Decoction on brain edema after intracerebral hemorrhage.

关 键 词：利开灵 脑出血 内毒素血症 炎症 肿瘤坏死因子-Α 白细胞介素-1Β 

分 类 号：R285.5[医药卫生—中药学]