维拉帕米抗戊四唑诱导小鼠惊厥的实验研究  

The anti-convulsion effect of verapamil on mice model induced by pentylenetetrazol

作　　者：王珣[1] 柳佳利[1] 孙浩[1] 李勤[1] 刘洋[1] 林熙[2] 

机构地区：[1]暨南大学医学院临床医学系,广州510632 [2]暨南大学医学院医学药理学系,广州510632

出　　处：《中国临床保健杂志》2011年第4期398-400,共3页Chinese Journal of Clinical Healthcare

基　　金：国家自然基金资助项目(81000209);教育部科学技术研究重点项目(210255);暨南大学科研培育与创新基金项目(21609304)

摘　　要：目的研究维拉帕米抗惊厥的治疗效果,为临床治疗惊厥提供新方法。方法将实验小鼠60只分为模型对照组、硫酸镁(MgSO4)组和实验组(维拉帕米低、中、高剂量组),每组12只。预先给予相应的治疗药物之后,腹腔注射戊四唑制造惊厥模型;造模成功后观察并记录小鼠的惊厥发生时间、存活时间、惊厥发生率和死亡率等。结果与模型对照组相比,硫酸镁组和维拉帕米各剂量组惊厥发生所需时间均有所增加(P<0.01),硫酸镁组小鼠的存活时间显著延长且死亡率降低(P<0.01);维拉帕米各剂量组小鼠的存活时间延长(P<0.01),低、中剂量组死亡率明显降低(P<0.01)。结论低、中剂量维拉帕米对于戊四唑所致的惊厥模型有较好的疗效;高剂量维拉帕米不宜用于抗惊厥治疗。Objective To study the effect of verapamil on anti-convulsion and provide a new therapeutic method for convulsion.Methods Sixty mice were divided into 3 groups as follow,the blank group,MgSO4 group and experimental group（the low,middle,high dosage of verapamil）.Pentylenetetrazol was intraperitoneally injected into mice after medication to make the model of convulsion.The occurrence time of convulsion,survival time of mice,the incidence rate of convulsion and the mortality of mice were observed.Results Compared with the blank group,either of MgSO4 and verapamil（low,middle or high dosage） groups had longer occurrence time of convulsion.The group of MgSO4 had significantly longer survival time and lower mortality of mice（P〈0.01）.The groups of verapamil（low,middle or high dosage） had also longer living time of mice（P〈0.01） and the lower mortality in the low and middle dosage groups（P〈0.01）.Conclusion The low or middle dosages of verapamil had a better curative effect of anti-convulsion in the convulsion model mice induced with pentylenetetrazol,but the high dosage of verapamil was not suitable for the anti-convulsion therapy.

关 键 词：发作 维拉帕米 戊四唑 硫酸镁 模型 动物 

分 类 号：R965[医药卫生—药理学]