香兰素衍生物VND3207对不同LET辐射致质粒DNA损伤的防护作用  

Protection of vanillin derivative VND3207 on plasmid DNA damage induced by different LET ionizing radiation

作　　者：徐辉辉[1] 汪黎[1] 隋丽[2] 关华[1] 王豫[1] 刘晓丹 张士猛[1] 徐勤枝[1] 王潇[2] 周平坤[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]中国原子能科学研究院

出　　处：《中华放射医学与防护杂志》2011年第4期416-419,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：基金项目：国家重点基础研究发展计划973项目（2007CB914603）;国家自然科学基金杰出青年基金（30825011）;国家自然科学基金青年基金（10805078）

摘　　要：目的评价不同LET辐射致DNA损伤以及药物的防护作用。方法分别以^60Coγ射线、质子束、^7Li重离子束照射溶液状态超螺旋构象质粒DNA，通过琼脂糖凝胶电泳分析DNA分子构象变化，检测DNA损伤程度及VND3207的防护作用。结果质子和^7Li重离子体外所致质粒DNA损伤明显比γ射线严重。药物VND3207能够有效减轻所观察的3种不同LET辐射对质粒DNA的损伤，加药组与不加药组相比，质粒DNA开环构象显著减少，保护效果随着药物浓度的增加更加明显。200μmol／L浓度对50Gyγ射线、质粒和^7Li重离子辐照质粒DNA损伤的保护效果（质粒超螺旋构象百分比）分别为85．3％（t=3．70，P=0．033）、73．3％（t=10．58，P=0．017）和80．4％（t=8．57，P=0．008）。可见VND3207对重离子辐射损伤的保护效应尤为显著，明显优于质子辐照损伤。结论药物VND3207对辐射DNA损伤具有较好的保护作用，特别是对．y射线和重离子辐射损伤的保护作用更为明显。Objective To evaluate the radioprotective effect of vanillin derivative VND3207 on DNA damage induced by different LET ionizing radiation. Methods The plasmid DNA in liquid was irradiated by ^60Co γ-rays, proton or ^7Li heavy ion with or without VND3207. The conformation changes of plasmid DNA were assessed by agarose gel electrophoresis and the quantification was done using gel imaging system. Results The DNA damage induced by proton and ^7Li heavy ion was much more serious as compared with that by ^60Co γ-rays, and the vanillin derivative VND3207 could efficiently decrease the DNA damage induced by all three types of irradiation sources, which was expressed as a significantly reduced ratio of open circular form （OC） of plasmid DNA. The radioprotective effect of VND3207 increased with the increasing of drug concentration. The protective efficieneies of 200 μmol/L VND3207 were 85.3%（t=3.70,P=0.033）, 73.3%（t=10.58, P=0.017）and 80.4%（t=8.57,P=0.008）on DNA damage induction by 50 Gy of γ-rays, proton and 7Li heavy ion, respectively. It seemed that the radioprotection of VND3207 was more effective on DNA damage induced by high LET heavy ion than that by proton. Conclusions VND3207 has a protective effect against the genotoxicity of different LET ionizing radiation, especially for γ-rays and 7 Li heavy ion.

关 键 词：香兰素衍生物 DNA损伤 辐射防护 Γ射线 重离子 质子 

分 类 号：R96[医药卫生—药理学]