血小板源性生长因子促进移植真皮多能干细胞向全身照射大鼠骨髓的分布  

Enhancement of distribution of dermal multipotent stem cells to bone marrow in rats of total body irradiation by platelet-derived growth factor-AA treatment

作　　者：宗兆文[1] 任永川[1] 沈岳[1] 陈永华[1] 冉新泽[2] 史春梦[2] 程天民[2] 

机构地区：[1]第三军医大学大坪医院全军创伤中心创伤科,重庆400042 [2]第三军医大学全军复合伤研究所国家创伤、烧伤与复合伤重点实验室

出　　处：《中华放射医学与防护杂志》2011年第4期433-436,共4页Chinese Journal of Radiological Medicine and Protection

基　　金：基金项目：创伤烧伤与复合伤国家重点实验室自主课题（SKLZZ200817）;重庆市自然科学基金重点项目（CSTC,2009BA5017）

摘　　要：目的观察血小板源性生长因子-AA（PDGF．AA）处理能否增加真皮多能干细胞（dMSCs）向全身照射（TBI）大鼠骨髓的分布。方法分离雄性大鼠dMSCs，向第3代dMSCs中加入10μg／LPDGF—AA，继续培养2h后，Westernblot检测dMSCs中tenascin—C的表达，Transwell小室观察dMSCs的迁移能力，并收集细胞静脉移植到雌性全身照射大鼠体内，伤后2周采用针对Y染色体的实时定量PCR法检测骨髓中dMSCs含量。以未处理的dMSCs作为对照。结果与未处理的dMSCs相比，PDGF—AA处理可上调dMSCs中tenascin—C的表达，在骨髓提取液的趋化下迁移到Transwell小室下层的细胞多，移植后分布到骨髓的dMSCs数量为（1．79±0．13）×10^5个，明显高于未处理的（1．24±0．09）×10^5个（t=8．833，P〈0．01）。结论移植前用PDGF-AA处理dMSCs可增强其迁移能力，并可增加其向全身照射大鼠骨髓的分布。Objective To observe whether dermal muhipotent stem cells （dMSCs） treated with platelet-derived growth factor-AA （PDGF-AA）could distribute more frequently to the bone marrow in rats of total body irradiation （TBI）. Methods Male dMSCs were isolated and 10 μg/L PDGF-AA was added to the culture medium and further cultured for 2 h. Then the expression of tenascin-C were examined by Western blot, and the migration ability of dMSCs was assessed in transwell chamber. The pre-treated dMSCs were transplanted by tail vein injection into female rats administered with total body irradiation, and 2 weeks after transplantation, real-time PCR was employed to measure the amount of dMSCs in bone marrow. Non-treated dMSCs served as control. Results PDGF-AA treatment increased the expression of tenascin-C in dMSCs, made （1.79 ± 0. 13） ×10^5 cells migrate to the lower chamber under the effect of bone marrow extract, and distributed to bone marrow in TBI rats, significantly more than （ 1.24± 0. 09） × 10^5 in non-treated dMSCs （t = 8. 833, P 〈 0.01）. Conclusions PDGF-AA treatment could enhance the migration ability of dMSCs and increase the amount of dMSCs in bone marrow of TBI rats after transplantation.

关 键 词：放射损伤 真皮多功能干细胞 血小板源性生长因子-AA 

分 类 号：R818[医药卫生—放射医学]