益气养阴消癥通络中药对高糖联合AngⅡ培养的大鼠系膜细胞p38MAPK信号通路的影响  

Effects of Supplementing Qi-Nourishing Yin-Dispersing Blood Stasis-Dredging Collateral Herbs on p38MAPK Signal Transduction Pathway in Rat Mesangial Cell Cultured by High Glucose and AngⅡ

作　　者：赵雯红[1] 陈志强[1] 张江华[2] 孙玉凤[3] 王月华[1] 王凤丽[1] 

机构地区：[1]河北省中医院肾内科,石家庄050011 [2]河北医科大学中西医结合研究所,石家庄050017 [3]河北医科大学第二医院消化内科,石家庄050000

出　　处：《中国中西医结合肾病杂志》2011年第8期669-672,754,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：河北省自然科学基金资助项目(No.C2008001074);河北省卫生厅基金资助项目(No.08165);河北省科技计划项目(No.09276101D-27)

摘　　要：目的:探讨益气养阴消癥通络中药对高糖联合血管紧张素Ⅱ培养的大鼠肾小球系膜细胞(MCs)p38MAPK信号通路的作用。方法:原代培养MCs,第5代时,予高糖、高糖联合血管紧张素Ⅱ刺激,并同时进行药物血清干预,48h后ELISA法检测细胞上清ColⅣ蛋白含量,Western blot检测MCs p-p38MAPK、p38MAPK、p-STAT3、STAT3蛋白表达水平。结果:中药血清可显著减少细胞上清ColⅣ蛋白含量(P<0.01),且能明显抑制p38MAPK、STAT3蛋白磷酸化水平(P<0.01)。结论:益气养阴消癥通络中药可能是通过下调p38MAPK信号通路表达,减少细胞外基质合成,从而发挥对肾脏的保护作用。Objective：To discuss the effects of supplementing qi - nourishing yin and disperse blood stasis - dredge collateral herbs on p38MAPK signal transduction pathway in rat mesangial cells Cultured by high glucose and AngⅡ.Methods：Rat mesangial cells were cultured from the first generation,stimulated by high glucose and high glucose combined with AngⅡat the fifth generation,and interfered with the medicine blood serum at the same time.After 48h,the content of ColIV,p-p38MAPK,p38MAPK,p-STAT3 and STAT3 protein was detected by ELISA and western blot respectively.Results：the herbs blood serum can decrease the content of ColIV protein and suppressed the phosphorylation of p38 MAPK and STAT3 significantly（P0.01）.Conclusion：Supplementing qi - nourishing yin and disperse blood stasis - dredge collateral herbs could protect the kidney by inhibiting the expression of p38 MAPK signal transduction pathway and synthesis of extracellular matrix.

关 键 词：益气养阴消癥通络中药 大鼠肾脏系膜细胞 P38MAPK信号通路 

分 类 号：R285.5[医药卫生—中药学]