内皮祖细胞对大鼠睾丸扭转复位后生精功能的影响  

Effects of endothelial progenitor cells on spermatogenic functions in testicular detorsion in rats

作　　者：曹朴[1,2] 薛建新[2,3] 贾瑞鹏[3] 许露伟[3] 李文成[3] 钱铭[3] 陈波[3] 薄成佳[3] 

机构地区：[1]东南大学医学院外科学系,南京210009 [2]东南大学附属第二医院外科 [3]南京医科大学附属南京第一医院泌尿外科

出　　处：《中华医学杂志》2011年第30期2135-2138,共4页National Medical Journal of China

基　　金：国家自然科学基金（81070597）;南京市医学重点科技发展项目（ZKX10003）志谢东南大学医学院余磊的帮助

摘　　要：目的探讨内皮祖细胞（EPC）移植对睾丸扭转复位后生精功能的影响。方法获取大鼠骨髓源性EPC，采用携带增强型绿色荧光蛋白的重组腺病毒（Ad．eGFP）转染EPC。将大鼠分3组，每组各6只。假手术组：左侧睾丸未扭转；缺血再灌注损伤（IRI）组：睾丸扭转复位后经股静脉注射1ml生理盐水；EPC组：睾丸扭转复位后注射1mlEPC悬液（EPC数为1．0×10^6个）。另外对3只睾丸扭转复位后大鼠进行Ad—eGFP转染后的EPC移植。术后5d，观察移植EPC的归巢特征，并检测各组睾丸组织病理变化以及凋亡细胞／生精小管情况。结果重复感染倍数（MOI）=50时，Ad—eGFP对EPC的转染效率〉73．7％。Ad-eGFPEPC移植后5d，可在左侧睾丸组织中发现绿色荧光细胞。假手术组生精细胞结构正常，凋亡细胞／生精小管为0．09±0．02。IRI组生精细胞稀少，凋亡细胞／生精小管为2．82±0．81。EPC组生精上皮较IRI组显著增厚，EPC组凋亡细胞／生精小管为0．32±0．09，低于IRI组，差异有统计学意义（P〈0．01）。结论EPC移植可改善睾丸扭转导致的生精功能障碍，对生精细胞产生保护作用。Objective To investigate the effects of transplanted endothelial progenitor cells （EPCs） on the spermatogenic functions in testicular detorsion. Methods Bone-marrow-derived EPCs were obtained from rats and transfected by enhanced green fluorescent protein adenovirus （Ad-eGFP）. The rats were divided into 3 groups （n = 6 each）. In the sham group, left testis was not twisted. In the ischemia reperfusion injury （IRI） group, 1 ml saline was injected into the femoral vein of each rat after testicular detorsion. In the EPCs group, 1 ml EPCs suspension （ 1.0 × 10^6 EPCs） was injected into each rat after testicular detorsion. The Ad-eGFP transfected EPCs were injected into the 3 additional rats of testicular torsion-detorsion. At Day 5 post-transplantation, the characteristics of transplanted EPCs homing were detected. And the pathological changes and apoptotic cells/seminiferous tubules in left testis were examined. Results When the value of multiplication of infection （MOI） was at 50, the transfection rate of EPCs by Ad- eGFP exceeded 73.7%. At Day 5 post-treatment, the cells exhibiting green fluorescence were detected in left testis. The germ cells in rats of the sham group were normal. And the ratio of apoptotic cells to seminiferous tubules was 0. 09± 0. 02. The germ cells in rats of the IRI group were much fewer. And the ratio of apoptotic cells to seminiferous tubules was 2. 82 ±0. 81. As compared with the IRI group, seminiferous epithelium was thicker in the EPCs group. And the ratio of apoptotic cells to seminiferous tubules was 0. 32 ± 0. 09 in the EPCs group. It was much smaller than that in the IRI group. There was significant difference （P 〈 0. 01 ）. Conclusion The transplantation of EPCs is effective for treating the spermatogenic dysfunctions caused by testicular torsion so as to greatly enhance the spermatogenic functions.

关 键 词：精索扭转 再灌注损伤 干细胞 细胞移植 增强型绿色荧光蛋白 

分 类 号：R6[医药卫生—外科学]