辐照花粉授粉及胚挽救时期对苦瓜离体雌核发育的影响  

Effects of Irradiated Pollen Pollination and Stages of Embryo Rescue on Embryo Development in vitro of Bitter Gourd

作　　者：陈小凤[1] 黄如葵[1] 黄玉辉[1] 刘杏连[1] 梁家作[1] 周生茂[1] 

机构地区：[1]广西农业科学院蔬菜研究所,广西南宁530007

出　　处：《北方园艺》2011年第16期152-154,共3页Northern Horticulture

基　　金：教育部留学回国人员科研启动基金;广西自然科学基金资助项目(桂科自0832086)

摘　　要：采用辐照花粉授粉结合胚挽救技术,以7个不同基因型苦瓜作花粉供体对MC52进行授粉,系统探讨辐照剂量、胚挽救时期对成苗率的影响及辐照剂量对产胚类型的影响。结果表明:进行苦瓜辐照花粉授粉诱导离体雌核发育的适合辐照剂量为150Gy,胚最佳抢救时期为授粉后第10天。试验发现辐照剂量在200Gy时也可获得高的坐果率,但大部分种子已成空壳,对于苦瓜辐照花粉授粉诱导雌核发育获得单倍体是没有意义的;对辐照获得的102株苗进行倍性鉴定,虽未得到单倍体植株,但在该试验中仍有较高比例的各种类型胚未发育成植株,还须进一步优化胚的培养条件。The technology of combining irradiated pollen pollination with embryo rescue were adopted,seven different bitter melon were used to maternal parents were paternal parent MC52 to systematically investigate the effects of irradiation doses,embryo rescue period on plantlets and the effects of irradiation doses on embryo types.The results showed that the 150 Gy dose was the best irradiation dose and the tenth day was the best embryo rescue period to induction of female gametogenesis for bitter melon.200 Gy dose could obtain high fruit rated,but it make no significant to product haploid for bitter melon,as the seeds became empty.The 102 plantlets were not had haploid through identified chromosome ploidy,but the high percent type embryos were not grew plantlets in the experiment,so culture conditions of embryo needed to improve.

关 键 词：苦瓜 辐照花粉 胚胎挽救 单倍体 

分 类 号：S642.5[农业科学—蔬菜学] Q691.8[农业科学—园艺学]