野生天门冬遗传多样性的ISSR分析  

ISSR Analysis of Genetic Diversity of Asparagus cochinchinensis

作　　者：欧立军[1,2,3] 王俞人[1] 刘佳蒙[1] 王敏[1] 

机构地区：[1]怀化学院生命科学系,湖南怀化418000 [2]怀化学院民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418000 [3]怀化学院湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008

出　　处：《北方园艺》2011年第16期164-166,共3页Northern Horticulture

基　　金：湖南省高校创新平台开放基金资助项目(09K106)

摘　　要：通过ISSR技术对19个野生天门冬居群共67个个体进行遗传多样性分析。结果表明:用13个随机引物共扩增出125条清晰条带,其中92条具多态性,平均多态性位点比率为73%,建立了不同居群的ISSR标记;Nei’s基因多样性指数H=0.19,Shannon’s多样性指数I=0.30,遗传分化指数Gst=0.8206。聚类分析表明,青海、云南和贵州省的5个居群聚为一大支,其它居群为另一大支。野生天门冬种内具有较高的遗传多样性,遗传变异主要存在于居群间;ISSR标记可以作为研究天门冬遗传多样性及居群鉴定的有效标记。The genetic diversity of 67 individuals from 19 populati-ons was analyzed by ISSR.The results showed that 13 primers were selected to produce highly reproducible ISSR bands,92 showed polymorphism among 125 amplified bands and the percentage of polymorphic bands reached 73%,ISSR marker of different populations was found.Nei＇ s gene diversity index（H） was 0.192,Shannon＇s information index was 0.30,genetic differentiation index was 0.8206.The Clustering tree showed the populations of Qinghai and Yunnan province,5 populations of Guizhou province were a branch,others populations were another branch.Asparagus had a high genetic diversity and the majority of genetic variation occurred among populations.The ISSR marker could be used for analysis of genetic diversity and identification of genetic variation of Asparagus cochinchinensis.

关 键 词：天门冬 居群 ISSR 遗传多样性 

分 类 号：S567.239[农业科学—中草药栽培]