两面针总生物碱HPLC指纹图谱研究  

HPLC Fingerprint of Total Alkaloid in Zanthoxylum nitidum

作　　者：刘华钢[1] 冯看[2] 叶月华 雷欣潮[4] 徐宏珍[4] 

机构地区：[1]广西医科大学,广西南宁530021 [2]柳州市疾病预防控制中心,广西柳州545007 [3]广西壮族自治区食品药品检验所,广西南宁530022 [4]广西中医学院,广西南宁530001

出　　处：《中国中医药信息杂志》2011年第9期62-64,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(30760302);广西壮族自治区科技厅自然科学基金(0832127)

摘　　要：目的对两面针总生物碱进行高效液相色谱法(HPLC)指纹图谱的研究,为科学评价及有效控制两面针质量提供可靠方法。方法采用HPLC梯度洗脱进行两面针总生物碱指纹图谱的研究,测定了33批两面针药材。色谱柱:UltimateTM×BC8柱(5μm,4.6 mm×250 mm);流动相:乙腈-水相(0.2%磷酸+0.2%三乙胺);分析时间:45 min;柱温:35℃;检测波长:250 nm。结果 HPLC指纹图谱标定了24个共有特征峰。结论方法学考察结果表明指纹图谱精密度较高,24 h稳定性较好,系统重复性良好,说明本法能有效控制两面针质量,为完善其质量评价提供依据。Objective To establish chromatographic fingerprint of total alkaloid in Zanthoxylum niridum by HPLC, thus provides method for the evaluation and quality control. Methods HPLC-UV method was applied to establish the chromatographic fingerprint of total alkaloid in Zanthoxylum niridum. The separation was performed on a Ultimate^TM ×BCs column （250 mm×4.6 mm, 5 μm） gradient eluted composed of acetonitrile and 0.2% phosphate acid and 0.2% triethylamine. The column temperature was set at 35 ℃and the flow rate was 1.0 mL/min. The detective wavelength was 250 nm. The HPLC fingerprint for 33 batches of Zanthoxylum niridum was studied on their similarity. Results Total of 24 characteristic peaks were established in the fingerprint. Conclusion This method is simple and accurate with good reproducibility, and can be used specifically for the quality control of Zanthoxylum niridum （Roxb.） DC.

关 键 词：两面针 总生物碱 高效液相色谱 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]