D_(101)大孔树脂分离茜草中大叶茜草素的研究  

Separation of Mollugin from Dried Rhizome of Rubia cordifolia L. with D_(101) Macroporous Resin

作　　者：谭朝阳[1] 姚宇[1] 王建华[1] 尤昭玲[1] 

机构地区：[1]湖南中医药大学中药药性与药效实验室,湖南长沙410208

出　　处：《中国中医药信息杂志》2011年第9期65-66,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家中医药管理局新药开发专项基金(D1X046B)

摘　　要：目的探讨D101树脂对大叶茜草素的分离方法。方法通过采用HPLC测定大叶茜草素含量,考察D101树脂对茜草提取液中大叶茜草素的吸附及解吸条件。结果可采用茜草的70%乙醇提取液直接上样进行吸附,并用95%乙醇洗脱,干燥后固形物中大叶茜草素的纯度达73.5%。结论 D101树脂适合用于分离大叶茜草素。分离工艺简便,可用于工业化生产。Objective To research the separation procedure of mollugin with D101 macroporous resin. Methods HPLC was used to analyze the content of mollugin, in order to study the conditions of adsorption and desorption of D101 macroporous resin. Results The 70% ethanol extract from dried rhizome of Rubia cordifolia L. was directly adsorbed by D101 macroporous resin and 95% ethanol as the eluant. The purity of mollugin was up to 73.5% after purification. Conclusion D101 macroporous resin is a good material for the separation of mollugin. The process is convenient and can be used for industrial production.

关 键 词：D101大孔树脂 茜草 大叶茜草素 纯化 

分 类 号：R283.5[医药卫生—中药学]