骆驼蓬灵单独和与洗必泰联合对抗金黄色葡萄球菌悬浮菌和生物膜的抗菌活性研究  

Antimicrobial Efficacy of Harmaline Alone and in Combination with Chlorhexidine Digluconate against Clinical Isolates of Staphylococcus aureus Grown in Planktonic and Biofilm Cultures

作　　者：申凤鸽[1] 邢明勋[1] 刘丽慧[1] 袁鹏[1] 史祺云[1] 于录[1] 

机构地区：[1]吉林大学人兽共患病研究所,长春130062

出　　处：《湖北农业科学》2011年第16期3346-3350,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金(30871889);高等学校博士学科专项科研基金(SRFDP)(200801831051);吉林省科技发展计划项目(200705233);中央高校基本科研业务费项目

摘　　要：采用微量稀释法测骆驼蓬灵和洗必泰(CHG)单独抗金黄色葡萄球菌悬浮菌和生物膜的最小抑菌浓度(MIC)和最小杀菌浓度(MBC);棋盘稀释法测定两种药物联合时的协同效果;以及激光共聚焦采集图片检测部分联合的效果。结果表明,骆驼蓬灵和CHG联合具有协同抗菌效果。在悬浮菌试验中,9株显示抗浮游菌协同活性,在生物膜试验中,11株显示协同抗生物膜活性。其余菌株显示相加活性,本试验中无颉颃作用。初步探索了体外试验协同作用的原因,为克服金黄色葡萄球菌耐药提供了一个可供选择的方法。Minimum inhibitory concentrations（MIC） and minimum bactericidal concentrations（MBC） of harmaline and chlorhexidine digluconate（CHG） against clinical isolates of Staphylococcus aureus were determined for each microorganism grown in suspension or biofilm using microbroth dilution method.Chequerboard assays were used to determine synergistic,indifferent or antagonistic interactions between harmaline and CHG;and some of the results were verified by confocal laser scanning microscopy.Harmaline and CHG showed effective antimicrobial activity against suspensions and biofilm cultures of S.aureus.Synergistic antimicrobial effects between harmaline and CHG were observed in 9 and 11 of the 13 S.aureus strains when in suspension and in biofilm,respectively.No antagonistic activity was observed in any of the strains tested.Synergistic activity of harmaline and CHG against clinical isolates of S.aureus（in suspension and in biofilm） were observed in vitro.This study might provide alternative methods to reduce the resistance of S.aureus both in suspension and biofilm.

关 键 词：骆驼蓬灵 洗必泰 协同作用 抗菌活性 

分 类 号：TQ464.4[化学工程—制药化工]