宁夏沙枣多糖的酶法提取工艺  

Study on Enzymatic Extracting Conditions of Polysaccharide from Elaeagnus angustifolia L. in Ningxia

作　　者：王凯[1] 王青[2] 

机构地区：[1]北方民族大学化学与化学工程学院,银川750021 [2]商丘医学高等专科学校,河南商丘476100

出　　处：《湖北农业科学》2011年第16期3368-3370,共3页Hubei Agricultural Sciences

基　　金：北方民族大学国家民委化工技术重点开放实验室项目(2009SY09)

摘　　要：研究酶法提取宁夏沙枣(Elaeagnus angustifolia L.)中多糖的提取工艺。在单因素试验的基础上,采用正交设计考察了不同pH值、酶的添加量、酸提时间对多糖提取率的影响。结果表明,最佳工艺条件为酶提pH值4.38,添加酶的质量分数5%,酶提时间3 h,酶提温度60℃。最佳条件下多糖的提取率为72.93%。The enzymatic extracting conditions of polysaccharide from Elaeagnus angustifolia L.in Ningxia was studied.Based on single factor test,the effects of pH,enzyme dosage,enzymolysis time on polysaccharide extracting rate were investigated and optimized by orthogonal test.The results showed that the optimum conditions for extracting polysaccharide from E.angustifolia by enzyme were pH 4.38,enzyme dosage 5%,enzymolysis time 3 h,enzymolysis temperature 60 ℃.Under optimal conditions,the extracting rate of polysaccharide was 72.93%.

关 键 词：宁夏沙枣 多糖 提取工艺 正交试验 

分 类 号：S665.1[农业科学—果树学]