黄芪提取物对乙型肝炎病毒抗原表达的抑制作用  

The Inhibitory Effect of Raidx Astragali Extract on HBV Antigen Secretion from HepG2.2.15 Cells

作　　者：胡芬[1] 杨泽天[1] 石铭俊[1] 杨占秋[1] 熊海蓉[1] 

机构地区：[1]武汉大学基础医学院医学病毒学研究所／病毒学国家重点实验室,湖北武汉430071

出　　处：《医学新知》2011年第4期254-256,共3页New Medicine

基　　金：国家重大新药创制专项资助（2009ZX09301-014-1）;中央高校基本科研业务费专项资金（4101045）;武汉大学大学生科研项目S2009363

摘　　要：目的探讨黄芪提取物对乙型肝炎病毒（HBV）病毒抗原表达的抑制作用。方法用MTT法测定黄芪提取液在不同浓度下对HepG2．2．15细胞的细胞毒性，ELISA法检测黄芪提取物对HepG2．2．15细胞分泌HBsAg、HBeAg颗粒的影响。结果黄芪提取物对HepG2．2．15细胞4d和8d的半数毒性浓度（TC50）分别为88．914μg／ml和85．209μg／ml（P〉0．05），对HBeAg4d和8d的治疗指数（TI）分别为1．018和1．342（P〉0．05），对HBsAg4d和8d的治疗指数分别为0．065和0．089（P〉0．05）。结论黄芪提取物抑制HepG2．2．15细胞分泌HBeAg的作用为低效有毒，黄芪提取物对HBsAg抗原分泌无明显抑制作用。Objective To explore the inhibitory effect of Raidx Astragali extract on HBV antigen secretion in HepG2.2.15 cells. Methods MTT colorimetric assay was employed to determine the cytotoxicity of Raidx Astragali extract . ELISA was used to evaluate the effect of various concentrations of Raidx Astragali extract on the heptitis B virus surface antigen（HBsAg） and Heptitis B virus e antigen（HBeAg） in the HepG2.2.15 cells. The inhibitory rate was calculated and therapeutic index（TI） was determined. Results 50% toxic concentration （TC50）at 4th day and 8th day of Raidx Astragali extract on HepG2.2.15 cell were 88.91μg/ml and 85.21 μg/ml ,respectively. The therapeutic index（TI） of at 4th day and 8th day of Raidx Astragali extract on HbsAg excretion were 0. 077 and 0. 425 （ P 〉 0.05 ）, respectively, and regarding to HBeAg, the TIs were 1. 302 and 1. 348 （ P 〉 0.05 ）. Conclusion Raidx Astragali extract show low effect with toxicity on inhibitory acitivity of HbeAg excretion and no obvious impact on HbsAg in HepG2.2.15 cell.

关 键 词：黄芪 HEPG2.2.15细胞 体外 HBV抗原 ELISA 

分 类 号：R373.21[医药卫生—病原生物学]