金黄色葡萄球菌生物膜形成与ica操纵子关系探讨  

Relationship between formation of Staphylococcus aureus biofilm and ica operon

作　　者：丁露[1] 黄小林[1] 陈开森[2] 廖晚珍[2] 胡雪飞[2] 胡妮娅[2] 

机构地区：[1]南昌大学公共卫生学院,2007级江西南昌330006 [2]南昌大学第一附属医院检验科,江西南昌330006

出　　处：《实验与检验医学》2011年第4期353-354,359,共3页Experimental and Laboratory Medicine

基　　金：南昌大学校级课题YF05(2008)

摘　　要：目的了解不同部位分离金黄色葡萄球菌在不同时间生物膜形成与ica操纵子关系。方法采用结晶紫法测定不同部位在不同时间形成生物膜的含量;采用PCR法测定菌株是否含有ica基因。结果生物膜形成能力强的菌株则ica基因大部分为阳性。结论含有ica基因的金黄色葡萄球菌则生物膜形成的能力较强。Objective To understand the relationship between the biofilm formation of Staphylococcus aureus separated from the clinical specimens in different parts or time and ica operon.Methods The crystal violet method was performed to determine the content of biofilm formation in different parts or time;PCR method was used to determine whether strains contains ica genes.Results The strains possessing the stronger ability of biofilm formation were tested positive for most of the ica genes.Conclusion Biofilm formation ability is stronger in Staphylococcus aureus containing ica genes.

关 键 词：金黄色葡萄球菌 生物膜 ICA操纵子 

分 类 号：R344.14[医药卫生—基础医学] R378.11