生长因子促进转基因小鼠坐骨神经体外预变性的实验研究  

Growth Factors Promote the Pre-degeneration of Sciatic Nerves of the Transgenic Mice In Vitro:a Preliminary Study

作　　者：王晓盼[1] 沈尊理[1] 秦金保[1] 金羽青[1] 

机构地区：[1]上海交通大学医学院附属第一人民医院整形外科,上海市200080

出　　处：《组织工程与重建外科杂志》2011年第4期191-195,共5页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金(30872630)

摘　　要：目的探索一种简便高效的小鼠坐骨神经体外预变性的方法,以便在短期内获取大量高活性的雪旺细胞。方法选用绿色荧光蛋白(GFP)转基因的小鼠24只,随机分为A、B、C和D四组。A、B、C组切取双侧坐骨神经,分别置于DMEM、DMEM+10%FBS及含生长因子的雪旺细胞培养液中体外预变性1周,D组切断一侧坐骨神经体内预变性1周。1周后取材,行免疫组织学和细胞学方面的检测,比较各组获取的雪旺细胞量和纯度。结果 C组获得的雪旺细胞量及纯度较高,与D组的雪旺细胞相似。结论生长因子能促进小鼠坐骨神经的体外预变性,具有与体内预变性相似的效果,能在短期能获得大量雪旺细胞。Objective To explore a convenient and effective method of pre-degeneration of the sciatic nerves and obtain large amounts of pure Schwann cells within a short period in vitro. Methods Twenty-four green fluorescent protein （GFP） transgenic mice were divided into A, B, C and D four groups randomly. Bilateral sciatic nerves were harvested and then cultured in DMEM （group A）, DMEM＋10%FBS （group B）, and SCCM containing growth factors （Group C） for one week for pre-degeneration. In group D, unilateral sciatic nerves were cut off for pre-degenaration in vivo as the control group. Results Group C obtained large amounts of Schwann cells with high purity, similar with group D. Conclusion Growth factors can promote the pre-degeneration of sciatic nerves of the transgenic mice in vitro, similar with the effect of pre-degeneration in vivo, and can obtain large amounts of Schwann cells with high purity.

关 键 词：生长因子 预变性 坐骨神经 雪旺细胞 转基因小鼠 

分 类 号：Q813.11[生物学—生物工程]