脾虚对小鼠比目鱼肌形态和功能的影响  

Effects of spleen deficiency on configuration and function of soleus muscle in mice

作　　者：冯斑[1] 王慧平[1] 党凯[1] 薛玮[1] 高云芳[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2011年第4期645-649,共5页Journal of Northwest University（Natural Science Edition）

基　　金：国家自然科学基金资助项目(30770273);陕西省教育厅科研计划基金资助项目(07JK382);陕西省中医管理局2007年度中医药科学技术研究基金资助项目

摘　　要：目的探讨脾虚对小鼠比目鱼肌形态和功能的影响。方法采用力竭游泳加剥夺睡眠的方法塑造脾虚模型,运用免疫组织化学方法测定小鼠比目鱼肌Ⅰ,Ⅱ型肌纤维的横截面积及比例,用PowerLab系统测定比目鱼肌等张收缩和强直收缩的最大张力。结果脾虚小鼠的体重体温均明显降低,比目鱼肌横截面积变小,Ⅰ,Ⅱ型肌纤维比例无显著性变化,等张收缩张力和强直收缩张力明显下降,脏器指数均低于正常组。结论脾虚可导致小鼠比目鱼肌发生明显萎缩,肌肉收缩力量明显降低。Aim To observe the effect of spleen deficiency on the configuration and function of soleus. Methods The spleen deficiency model was established by swimming and sleep deprived; the cross sectional area （CSA） of type Ⅰ and Ⅱ fibers, relative proportion of type Ⅰ and Ⅱ fibers were measured by immunohistochemical method; and the peak isotonic twitch tension and maximum titanic tension of soleus were detected. Results Compared with the control group, the body weight, body temperature of deficiency of spleen group decrease; the CSA, peak isotonic twitch tension, maximum titanic tension of soleus reduce ; the transformation from type Ⅰ to type Ⅱ has no remarkable change ; the viscera index decline. Conclusion The deficiency of spleen can lead to remarkable soleus and muscle tension reduction. atrophy

关 键 词：脾虚 比目鱼肌 肌纤维横截面积 强直收缩 等张收缩 

分 类 号：Q445[生物学—生理学]