鲟鱼金属硫蛋白排铅作用及对锌含量影响的研究  

The effect of sturgeon-metallothionein(MT) on lead expelling and the zinc content in mouse

作　　者：张丁丁[1] 丁世彬[1] 廖丹[1] 兰泽桥 刘爱平[3] 李义勇 文左凤 应晨江[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉430030 [2]武汉天峡鲟都科技有限公司 [3]华中科技大学同济医学院公共卫生学院流行病与统计学系,武汉430030

出　　处：《微量元素与健康研究》2011年第5期1-3,共3页Studies of Trace Elements and Health

摘　　要：目的:探讨鲟鱼金属硫蛋白对铅中毒小鼠的排铅作用及其对锌含量的影响。方法:将昆明种小鼠20只随机分成正常组、铅模型组、阳性药物组(依地酸二钠钙)和金属硫蛋白组(以鲟鱼金属硫蛋白灌胃)。除正常组外,其它三组连续30 d自由饮用1.65%的醋酸铅水溶液。30 d后,测量脾脏系数,血液、股骨、睾丸中的铅和锌含量。结果:MT组小鼠血铅和股骨铅水平均明显低于铅模型组小鼠(P<0.05);MT组小鼠血锌水平均显著高于正常组和铅模型组(P<0.05),股骨锌含量低于正常组(P<0.05)。但未见MT对睾丸的铅、锌水平及氧化损伤保护有明显的影响。结论:鲟鱼MT具有促进排铅和拮抗铅中毒引起的锌含量下降的作用。Objective： To study the lead- expelling effects of sturgeon- metallothionein（MT） and its role on the zinc content sustaining in mouse. Methods： A total of 20 Kunming mouse were randomly divided into four groups： normal control, lead - exposure, lead - exposure with EDTA- treated, and lead - exposure with MT - treated groups. Except the normal control group, the mouse in other three groups exposed to lead via drinking lead acetate solution（ 1.65 96 ） freely. Thirty days later, organ coefficient of spleen, the lead and zinc content of blood, femur and testicle were measured. Results： The lead content of blood and normal control femur in NIT - treated group were significantly lower than those in lead- treated group （P〈 0.05 ）. Increased serum zinc content was observed in MT - treated group comparing with the normal control and lead - treated groups （ P 〈 0.05 ）. While decreased zinc content from femur was shown in MT- treated group comparing with the normal control group. No obvious effect of MT treatment on testicle lead and zinc content and protection from oxidative damage were observed. Conclusion： Sturgeon- metallothionein has the functions of lead- expelling and the zinc content sustaining during lead exposure.

关 键 词：鲟鱼金属硫蛋白 排铅 锌 小鼠 

分 类 号：R595.2[医药卫生—内科学]