葡萄糖酸铬对体外高糖培养乳鼠心肌细胞保护作用  

The Protect Effect of chromium gluconant on the cardiomyocytes in cultured neonate rat in glucose

作　　者：张杰[1] 杨旭东[1] 王春涛[1] 

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011

出　　处：《微量元素与健康研究》2011年第5期4-5,共2页Studies of Trace Elements and Health

基　　金：治疗胰岛素抵抗中草药筛选评价系统的建立及新药寻找(11511427)

摘　　要：目的:探讨葡萄糖酸铬对体外高糖培养乳鼠心肌细胞的保护作用及其机制。方法:Wistar乳鼠原代细胞随机分为空白对照组、高糖组及葡萄糖酸铬高、低剂量组。用荧光显微镜测定各组细胞凋亡情况。结果:经葡萄糖酸铬干预后,心肌细胞存活率明显增高,凋亡细胞明显减少,bcl-2表达增加,caspase-3表达减少。结论:葡萄糖酸铬抑制体外高糖培养的心肌细胞凋亡,增高细胞存活率,其机制可能与抑制caspase-3、激活bcl-2表达有关。Objective：To investigate the effect of chromium gluconant on glucose on the cardiomyocytes apopotosis in cultured neonate rat and its mechanism. Methods： Primitive cardiomyocytes of Wistar neonate rats were randomly divided into normal control group, the glucose group, 0.1 mg/mL chromium gluconant, 0.2 mg/mL chromium gluconant. Cell apoptosis was measured by fluorescence microscope and the levels of bcl - 2 and caspase - 3 gene expression were measured by RT - PCR. Results： chromium gluconant could inhibite the apoptosis of cardiomyocytes of Wistar neonate rats and improve the level of bcl - 2 gene expression and decrease the level of caspase- 3 gene expression. Conclusion： chromium gluconant inhibite the apoptosis of cardiomyocytes of Wistar neonate rats through down regulating bcl - 2 gene expression and up regulating caspase - 3 gene expression.

关 键 词：铬 糖尿病 细胞凋亡 BCL-2 CASPASE-3 

分 类 号：R587.1[医药卫生—内分泌]