白鹤灵芝不同提取部位对小鼠出血、凝血时间的影响  

Effects of different extraction of Rhinacanthus Nasutus(L.) Lindau on bleeding and coagulation time in mice

作　　者：谢丽莎[1] 蒙田秀[1] 周芳[1] 黄振园[1] 

机构地区：[1]广西中医学院,广西南宁530001

出　　处：《上海中医药杂志》2011年第9期66-67,共2页Shanghai Journal of Traditional Chinese Medicine

基　　金：广西壮族自治区自然科学基金资助项目(2010GENSFA013205)

摘　　要：目的观察白鹤灵芝不同提取部位对小鼠出血、凝血时间的影响。方法取正常健康小鼠110只,随机分为11组,分别为生理盐水组、丹参组(3.20g/kg)、白鹤灵芝三个提取部位的高(48.0g/kg)、中(24.0g/kg)、低(12.0g/kg)剂量组。每组10只,分别灌胃给药,连续7d。分别采用断尾法测定小鼠的出血时间,玻片法和玻管法测定小鼠的凝血时间。结果与生理盐水组比较,除正丁醇低剂量组外,其余各组小鼠出血时间延长,差异有统计学意义(P<0.05,P<0.01)。玻片法结果显示,与生理盐水组比较,石油醚与乙酸乙酯的高剂量组小鼠凝血时间延长,差异有统计学意义(P<0.01);丹参组,石油醚低剂量组,乙酸乙酯中剂量组,正丁醇高、中剂量组小鼠凝血时间延长,差异有统计学意义(P<0.05)。毛细血管法结果显示,与生理盐水组比较,石油醚低剂量组小鼠凝血时间延长,差异有统计学意义(P<0.01);丹参组,石油醚高、中剂量组,乙酸乙酯高、中剂量组,正丁醇高、中剂量组凝血时间延长,差异有统计学意义(P<0.05)。结论白鹤灵芝不同提取部位有延长小鼠出血时间和凝血时间,提示白鹤灵芝有一定活血化瘀的作用。Objective To observe the effect of different extraction of Rhinacanthus Nasutus （ L. ） Lindau on bleeding and coagulation time in mice. Methods The bleeding time was measured by using tail-cutting method, the coagulation time was measured by using the slice method and capillary method respectively. Results The bleeding and coagulation time was prolonged significantly by comparing with NS group. The high group of Mineral ether and Ethyl acetate had very sigificant differences（ P 〈 0.01 ）, other groups were of sigificant difterences（ P 〈 0.05 ）. Conclusion Different extraction of Rhinacanthus Nasutus （ L. ） Lindau can prolong bleeding and coagulation.

关 键 词：白鹤灵芝 不同提取部位 出血时间 凝血时间 

分 类 号：R285.5[医药卫生—中药学]