饲用羽毛肽粉中色氨酸的测定  

Determination of Tryptophan in Forage Feather Peptide Powder

作　　者：林香信[1,2] 姚清华[1,2] 宋永康[1,2] 

机构地区：[1]福建省农业科学院中心实验室,福建福州350003 [2]福建省精密仪器农业测试重点实验室,福建福州350003

出　　处：《畜牧与饲料科学》2011年第7期39-41,共3页Animal Husbandry and Feed Science

基　　金：福建省属公益类项目(2009R10034-1);福建省农业科学院青年基金(2009QB-20)

摘　　要：比较了多种碱解剂及碱解条件对饲用羽毛肽粉中色氨酸(Trp)测定的影响。结果表明,4mol/L LiOH、(110±1)℃水解20h效果最好,其次是6mol/L NaOH+3%SnCl2、(110±1)℃水解20h。并进一步探讨了测定中碱解剂用量、碱解温度、碱解管内空气、氨的浓度等对色氨酸测定值的影响。The effects of various alkali-hydro agents and alkali-hydrolyzable conditions on the determination of tryptophan in the forage feather peptide powder were compared. The results showed that the effect of hydrolyzing with 4 mol/L LiOH at（110±1） ℃ for 20 h was the best,the effect of hydrolyzing with 6 mol/L NaOH and 3% SnCl2 at（110±1） ℃ for 20 h was the next. The effects of the dose of alkali-hydro agent,alkali-hydrolyzable temperature,the concentration of atmosphere and ammonia in the alkali-hydrolyzable tube on the determined value of tryptophan in the determination were further discussed.

关 键 词：饲用羽毛肽粉 色氨酸 氨基酸自动分析仪 碱解剂 

分 类 号：S816.4[农业科学—饲料科学]