Transgenic Rice Plants Harboring Genomic DNA from Zizania latifolia Confer Bacterial Blight Resistance  

作　　者：SHEN Wei-wei SONG Cheng-li CHEN Jie FuYaping WU Jian-li JIANG Shao-mei 

机构地区：[1]College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China [2]China National Rice Research Institute, Hangzhou 310006, China [3]jiangxi University of Finance and Economics, Nanchang 330013, China

出　　处：《Rice science》2011年第1期17-22,共6页水稻科学（英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30760115);Transgenic Program (Grant No. 2008ZX08001-002)

摘　　要：Based on the sequence of a resistance gene analog FZ14 derived from Zizania latifolia （Griseb.）, a pair of specific PCR primers FZ14P1/FZ14P2was designed to isolate candidate disease resistance gene. The pooled-PCR approach was adopted using the primer pair to screen a genomic transformation-competent artificial chromosome （TAC） library derived from Z. latifolia. A positive TAC clone （ZR1） was obtained and confirmed by sequence analysis. The results indicated that ZR1 consisted of conserved motifs similar to P-loop （kinase la）, kinase 2, kinase 3a and GLPL （Gly-Leu-Pro-Leu）, suggesting that it could be a portion of NBS-LRR type of resistance gene. Using Agrobacterium-mediated transformation of Nipponbare mature embryo, a total of 48 independent transgenic To plants were obtained. Among them, 36 plants were highly resistant to the virulent bacterial blight strain PXO71. The results indicate that ZR1 contains at least one functional bacterial blight resistance gene.Based on the sequence of a resistance gene analog FZ14 derived from Zizania latifolia （Griseb.）, a pair of specific PCR primers FZ14P1/FZ14P2was designed to isolate candidate disease resistance gene. The pooled-PCR approach was adopted using the primer pair to screen a genomic transformation-competent artificial chromosome （TAC） library derived from Z. latifolia. A positive TAC clone （ZR1） was obtained and confirmed by sequence analysis. The results indicated that ZR1 consisted of conserved motifs similar to P-loop （kinase la）, kinase 2, kinase 3a and GLPL （Gly-Leu-Pro-Leu）, suggesting that it could be a portion of NBS-LRR type of resistance gene. Using Agrobacterium-mediated transformation of Nipponbare mature embryo, a total of 48 independent transgenic To plants were obtained. Among them, 36 plants were highly resistant to the virulent bacterial blight strain PXO71. The results indicate that ZR1 contains at least one functional bacterial blight resistance gene.

关 键 词：Zizania latifolia transformation-competent artificial chromosome library resistance-gene analog Oryza sativa bacterial blight resistance gene transfer 

分 类 号：S332.2[农业科学—作物遗传育种] Q781[农业科学—农艺学]