Transmission of Rice Black-Streaked Dwarf Virus from Frozen Infected Leaves to Healthy Rice Plants by Small Brown Planthopper (Laodelphax striatellus)  

作　　者：ZHou Tong Wu Li-juan WANG Ying CHENG Zhao-bang JI Ying-hua FAN Yong-jian Zuou Yi-jun 

机构地区：[1]Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China [2]These authors contributed equallyto this paper

出　　处：《Rice science》2011年第2期152-156,共5页水稻科学（英文版）

基　　金：supported by the National Basic Research Program(Grant No.2010CB126203);Special Fund for Agro-scientific Research in the Public Interest(Grant No. 201003031);the National Natural Science Foundation of Jiangsu Province,China(Grant Nos.BK2009325 and BK2010018)

摘　　要：In order to preserve virus for identifying the resistance of rice varieties against rice black-streaked dwarf disease, a simple and reliable method was developed, through which virus-free small brown planthopper （SBPH） acquired rice black-streaked dwarf virus （RBSDV） from frozen infected leaves and the virus was transmitted to healthy rice plants. The experimental results showed that SBPH could obtain RBSDV from frozen infected rice leaves and the virus could be transmitted to a susceptible rice variety. For the ability to acquire RBSDV and transmit the virus to healthy plants by SBPH, there was no significant difference between frozen infected leaves and in vitro infected leaves. The novel method could be applied to identification of rice variety resistance to rice black-streaked dwarf disease, facilitating the breeding process for rice black-streaked dwarf disease resistance.In order to preserve virus for identifying the resistance of rice varieties against rice black-streaked dwarf disease, a simple and reliable method was developed, through which virus-free small brown planthopper （SBPH） acquired rice black-streaked dwarf virus （RBSDV） from frozen infected leaves and the virus was transmitted to healthy rice plants. The experimental results showed that SBPH could obtain RBSDV from frozen infected rice leaves and the virus could be transmitted to a susceptible rice variety. For the ability to acquire RBSDV and transmit the virus to healthy plants by SBPH, there was no significant difference between frozen infected leaves and in vitro infected leaves. The novel method could be applied to identification of rice variety resistance to rice black-streaked dwarf disease, facilitating the breeding process for rice black-streaked dwarf disease resistance.

关 键 词：rice black-streaked dwarf virus frozen infected leaves small brown planthopper methodology 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] X503.1[农业科学—植物保护]